Early dynamic changes in iPSC oxygen consumption rate predict future cardiomyocyte differentiation

• Published in: Biotechnology and bioengineering Vol. 120; no. 8; pp. 2357 - 2362
• Main Authors: Nikitina, Arina A., Roysam, Tanya, Kemp, Melissa L.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.08.2023
• Subjects: Bioreactors; Cardiomyocytes; Cardiovascular diseases; Cell differentiation; cell manufacturing; critical quality attributes; induced pluripotent stem cells; machine learning; Manufacturing; Oxygen; Oxygen consumption; oxygen consumption rate; Oxygen probes; Pluripotency; Quality management; Stem cells; time‐series analysis; cell manufacturing; induced pluripotent stem cells; critical quality attributes; machine learning; oxygen consumption rate; time-series analysis

Human induced pluripotent stem cells (iPSCs) hold great promise for reducing the mortality of cardiovascular disease by cellular replacement of infarcted cardiomyocytes (CMs). CM differentiation via iPSCs is a lengthy multiweek process and is highly subject to batch‐to‐batch variability, presenting...