Multispecies biofilm removal by XP‐endo Finisher and passive ultrasonic irrigation: A scanning electron microscopy study

• Published in: Australian endodontic journal Vol. 48; no. 1; pp. 91 - 97
• Main Authors: Teves, Abel, Blanco, Daniel, Casaretto, Mario, Torres, John, Alvarado, Débora E., Coaguila‐Llerena, Hernán, Faria, Gisele, Jaramillo, David E.
• Format: Journal Article
• Language: English
• Published: Australia Wiley Subscription Services, Inc 01.04.2022
• Subjects: biofilm; Biofilms; Dental Pulp Cavity - microbiology; Electron microscopes; Endodontics; Irrigation; Lavage; Microscopy, Electron, Scanning; passive ultrasonic irrigation; Premolars; Root Canal Irrigants - therapeutic use; root canal treatment; Scanning electron microscopy; Sodium hypochlorite; Sodium Hypochlorite - therapeutic use; Therapeutic Irrigation - methods; Ultrasonics; Water; XP‐endo Finisher; XP-endo Finisher; scanning electron microscopy; passive ultrasonic irrigation; sodium hypochlorite; biofilm; root canal treatment
• ISSN: 1329-1947; 1747-4477
• DOI: 10.1111/aej.12549

The aim was to assess the effect of XP‐endo Finisher (XPF) on multispecies biofilm removal, in comparison with passive ultrasonic irrigation (PUI) and conventional syringe irrigation (CSI), by scanning electron microscope (SEM). Fifty mandibular first premolars were instrumented, longitudinally sectioned. The split halves were incubated for 4 days with a broth obtained from three bacteria strains: Enterococcus faecalis, Eikenella corrodens and Streptococcus anginosus. Subsequently, the re‐approximated split halves were irrigated with 4% sodium hypochlorite (NaOCl) or water using CSI, and the final irrigation protocols were CSI with 4% NaOCl (CSI+4%NaOCl), PUI+4%NaOCl, XPF+4%NaOCl and CSI+water. The analysis of biofilm removal was performed using SEM images. There were no differences between PUI and XPF (P > 0.05), and both groups promoted higher biofilm removal than CSI+4%NaOCl and CSI+water groups (P < 0.05). It can be concluded the multispecies biofilm removal was significantly improved using XPF and PUI when compared to CSI.