LncRNA–CDC6 promotes breast cancer progression and function as ceRNA to target CDC6 by sponging microRNA‐215

• Published in: Journal of cellular physiology Vol. 234; no. 6; pp. 9105 - 9117
• Main Authors: Kong, Xiaoli, Duan, Yi, Sang, Yuting, Li, Yaming, Zhang, Hanwen, Liang, Yiran, Liu, Ying, Zhang, Ning, Yang, Qifeng
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.06.2019
• Subjects: Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cancer; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cell division; Cell Movement; Cell Proliferation; Disease Progression; Ectopic expression; Female; G1 phase; G1 Phase Cell Cycle Checkpoints; Gene Expression Regulation, Neoplastic; Humans; lncRNA–CDC6; MCF-7 Cells; Metastases; Metastasis; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; miR‐215; Neoplasm Invasiveness; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; proliferation; Ribonucleic acid; RNA; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Signal Transduction; Tumors; breast cancer; miR-215; proliferation; metastasis; lncRNA-CDC6
• DOI: 10.1002/jcp.27587
• ISSN: 0021-9541; 1097-4652

Rapid proliferation and metastasis of breast cancers resulted in poor prognosis in clinic. Recent studies have proved that long noncoding RNAs (lncRNAs) were involved in tumor progression. In this study, we aimed to determine the roles and mechanisms of lncRNA–cell division cycle 6 (CDC6) in regulating proliferation and metastasis of breast cancer. Clinically, lncRNA–CDC6 was highly expressed in tumor tissues and was positively correlated with clinical stages of breast cancers. Functionally, the ectopic expression of lncRNA–CDC6 promoted proliferation via regulation of G1 phase checkpoint, and further promoting the migration capability. Moreover, lncRNA–CDC6 could function as competitive endogenous RNA (ceRNA) via directly sponging of microRNA‐215 (miR‐215), which further regulating the expression of CDC6. Taken together, our results proved that lncRNA–CDC6 could function as ceRNA and promote the proliferation and metastasis of breast cancer cells, which provided a novel prognostic marker for breast cancers in clinic. Clinically, long noncoding RNAs (lncRNA)–cell division cycle 6 (CDC6) was highly expressed in tumor tissues and was positively correlated with clinical stages of breast cancers. Functionally, ectopic expression of lncRNA–CDC6 promoted proliferation via regulation of G1 phase checkpoint, and further promoting the migration capability. Moreover, lncRNA–CDC6 could function as competitive endogenous RNA (ceRNA) via directly sponging of microRNA‐215 (miR‐215), which further regulating the expression of CDC6.