A novel approach to residence time distribution characterization in a mAb continuous process

• Published in: Biotechnology and bioengineering Vol. 118; no. 9; pp. 3486 - 3498
• Main Authors: Brantley, Tim, Bogue, Jon, Denny, Kurtis, Elouafiq, Sanaa, Madren, Seth, Nakhle, Bassam, Khattak, Sarwat
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.09.2021
• Subjects: Animals; Antibodies; Antibodies, Monoclonal - biosynthesis; ATF perfusion; Bioreactors; Cell size; Chinese hamster ovary (CHO); CHO Cells; Continuously stirred tank reactors; Cricetulus; Fouling; Glycan; Glycosylation; Inhibitors; Monoclonal antibodies; Perfusion; product sieving; Residence time distribution; product sieving; Chinese hamster ovary (CHO); residence time distribution; ATF perfusion

Residence time distribution modeling of integrated perfusion to capture process can elucidate the impact of product quality excursions and filter fouling on monoclonal antibody production. In this case study, a glycosylation inhibitor and fluorescently labeled antibody are applied to the continuous...