Methyl-Guanine-Methyl-Transferase Transgenic Bone Marrow Transplantation Allows N,N-bis(2-chloroethyl)-Nitrosourea Driven Donor Mixed-Chimerism Without Graft-Versus-Host Disease, and With Donor-Specific Allograft Tolerance

• Published in: Transplantation Vol. 99; no. 12; p. 2476
• Main Authors: Hu, Min, Kramer, Belinda, Zhang, Geoff Y, Wang, Yuan Min, Watson, Debbie, Howden, Brian, McCowage, Geoff, Alexander, Ian E, Gunning, Peter, Alexander, Stephen I
• Format: Journal Article
• Language: English
• Published: United States 01.12.2015
• Subjects: Animals; Antineoplastic Agents, Alkylating - pharmacology; Bone Marrow Transplantation; Carmustine - pharmacology; Disease Models, Animal; DNA Modification Methylases - metabolism; DNA Repair Enzymes - metabolism; Graft vs Host Disease - drug therapy; Graft vs Host Disease - immunology; Graft vs Host Disease - metabolism; Heart Transplantation; Immune Tolerance - immunology; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Inbred CBA; Skin Transplantation; Transplantation Chimera; Tumor Suppressor Proteins - metabolism

Transplant tolerance has been achieved by mixed chimerism in animal models and in a limited number of kidney transplant patients. However, these mixed-chimerism strategies were limited either by loss of long-term mixed chimerism or risk of graft-versus-host disease (GVHD). Selective bone marrow (BM)...