Parkin Maintains Robust Pacemaking in Human Induced Pluripotent Stem Cell‐Derived A9 Dopaminergic Neurons

• Published in: Movement disorders Vol. 38; no. 7; pp. 1273 - 1281
• Main Authors: Pu, Jiali, Lin, Lin, Jiang, Houbo, Hu, Zhixing, Li, Hong, Yan, Zhen, Zhang, Baorong, Feng, Jian
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.07.2023; Wiley Subscription Services, Inc
• Subjects: A9 dopaminergic neurons; Calcium channels; CRISPR; Dopamine receptors; Gene targeting; induced pluripotent stem cells; Inhibitory postsynaptic potentials; isogenic; Movement disorders; Mutation; Neurodegeneration; Neurodegenerative diseases; Neurons; Nuclease; pacemaking; Parkin protein; Parkinson's disease; Pluripotency; Stem cells; Substantia nigra; Transcription activator-like effector nucleases; A9 dopaminergic neurons; isogenic; induced pluripotent stem cells; Parkinson's disease; pacemaking

Background The degeneration of nigral (A9) dopaminergic (DA) neurons results in cardinal motor symptoms that define Parkinson's disease (PD). Loss‐of‐function mutations in parkin are linked to a rare form of early‐onset PD that is inherited recessively. Objective We generated isogenic human A9...