A Reactive Oxygen Species–Mediated Loop Maintains Increased Expression of NADPH Oxidases 2 and 4 in Skin Fibroblasts From Patients With Systemic Sclerosis

• Published in: Arthritis & rheumatology (Hoboken, N.J.) Vol. 67; no. 6; pp. 1611 - 1622
• Main Authors: Spadoni, Tatiana, Svegliati Baroni, Silvia, Amico, Donatella, Albani, Lisa, Moroncini, Gianluca, Avvedimento, Enrico V., Gabrielli, Armando
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.06.2015
• Subjects: Adult; Aged; Aged, 80 and over; Cells, Cultured; Deoxyribonucleic acid; DNA; DNA damage; DNA repair; Female; Fibroblasts - metabolism; Fluorescent Antibody Technique; Fluorometry; Gene Silencing; Humans; Immunoblotting; Male; Medical research; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Microscopy, Confocal; Middle Aged; NADPH Oxidase 2; NADPH Oxidase 4; NADPH Oxidases - genetics; NADPH Oxidases - metabolism; Oxygen; Reactive Oxygen Species; Real-Time Polymerase Chain Reaction; RNA, Messenger - metabolism; RNA, Small Interfering; Scleroderma; Scleroderma, Systemic - genetics; Scleroderma, Systemic - metabolism; Skin - cytology; Skin - metabolism

Objective Reactive oxygen species (ROS) contribute to the pathogenesis of fibrosis in systemic sclerosis (SSc; scleroderma), and NADPH oxidase (NOX) is an important source of ROS. Since the role of single NOX isoforms has not been previously investigated in SSc, this study was undertaken to assess t...