Using laser capture microdissection to study fiber specific signaling in locomotor muscle in COPD: A pilot study

• Published in: Muscle & nerve Vol. 55; no. 6; pp. 902 - 912
• Main Authors: Mohan, Divya, Lewis, Amy, Patel, Mehul S., Curtis, Katrina J., Lee, Jen Y., Hopkinson, Nicholas S., Wilkinson, Ian B., Kemp, Paul R., Polkey, Michael I.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.06.2017
• Subjects: Adult; Aged; Aged, 80 and over; Biopsy; Case-Control Studies; Chronic obstructive pulmonary disease; Degradation; fiber type; Fibers; Gene expression; Gene Expression Regulation - physiology; Humans; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - metabolism; Laser Capture Microdissection; Lung diseases; Male; microdissection; Middle Aged; Muscle Fibers, Skeletal - classification; Muscle Fibers, Skeletal - metabolism; Muscle Fibers, Skeletal - pathology; Muscle Proteins - genetics; Muscle Proteins - metabolism; muscle weakness; Muscle, Skeletal - physiopathology; Myosin Heavy Chains - metabolism; Obstructive lung disease; Phosphotransferases (Alcohol Group Acceptor) - genetics; Phosphotransferases (Alcohol Group Acceptor) - metabolism; Pilot Projects; Polymerase chain reaction; Protein biosynthesis; Protein synthesis; Pulmonary Disease, Chronic Obstructive - pathology; Quadriceps muscle; Signal Transduction - physiology; SKP Cullin F-Box Protein Ligases - genetics; SKP Cullin F-Box Protein Ligases - metabolism; Statistics, Nonparametric; Temperature effects; fiber type; humans; microdissection; muscle weakness; quadriceps muscle

ABSTRACT Introduction Quadriceps dysfunction is important in chronic obstructive pulmonary disease (COPD), with an associated increased proportion of type II fibers. Investigation of protein synthesis and degradation has yielded conflicting results, possibly due to study of whole biopsy samples, whe...