CK1 inhibitor affects in vitro maturation and developmental competence of bovine oocytes

• Published in: Reproduction in domestic animals Vol. 54; no. 8; pp. 1104 - 1112
• Main Authors: Shi, Pengfei, Xu, Jie, Zhao, Xin, Shen, Penglei, Wen, Dongmei, Yu, Qing, Deng, Yanfei, Shi, Deshun, Lu, Fenghua
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.08.2019
• Subjects: Animals; Bax protein; Benzamides - pharmacology; Casein; casein kinase 1; Casein kinase I; Casein Kinase I - antagonists & inhibitors; Casein Kinase I - metabolism; Cattle; Connexin 43; D4476; Embryo Culture Techniques; Embryo, Mammalian - physiology; Embryonic Development; Fertilization in Vitro - drug effects; Fertilization in Vitro - veterinary; Gametocytes; Imidazoles - pharmacology; In vitro fertilization; in vitro maturation; In Vitro Oocyte Maturation Techniques - methods; In Vitro Oocyte Maturation Techniques - veterinary; Kinases; Male; MAP kinase; Maturation; Meiosis; Morphology; oocyte; Oocytes; Oocytes - physiology; Proteins; Staining; Wnt/β‐catenin; casein kinase 1; in vitro maturation; oocyte; D4476; Wnt/β-catenin

The objectives of present study were to evaluate the effect of casein kinase 1 (CK1) inhibition D4476 on in vitro maturation (IVM) and developmental competence of bovine oocytes. The cumulus oocyte complexes (COCs) were cultured in maturation medium with D4476 (0, 2, 5, 10, 20 μM) for 24 hr. After I...