Stapled NRPS enhances the production of valinomycin in Escherichia coli

• Published in: Biotechnology and bioengineering Vol. 120; no. 3; pp. 793 - 802
• Main Authors: Huang, Shuhui, Ba, Fang, Liu, Wan‐Qiu, Li, Jian
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.03.2023
• Subjects: Anti-Bacterial Agents; Biocompatibility; Biosynthesis; Biosynthetic Pathways; E coli; enzyme engineering; Enzymes; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Metabolites; Natural products; Natural resources; nonribosomal peptide; NRPS; Optimization; Peptide Synthases - genetics; Peptide Synthases - metabolism; Peptides; Peptides - metabolism; Secondary metabolites; strain and bioprocess optimization; synthetic biology; Toxicity; Valinomycin; Valinomycin - metabolism; enzyme engineering; synthetic biology; NRPS; valinomycin; strain and bioprocess optimization; nonribosomal peptide

Nonribosomal peptides (NRPs) are a large family of secondary metabolites with notable bioactivities, which distribute widely in natural resources across microbes and plants. To obtain these molecules, heterologous production of NRPs in robust surrogate hosts like Escherichia coli represent a feasibl...