Relocation of NPM Affects the Malignant Phenotypes of Hepatoma SMMC‐7721 Cells

• Published in: Journal of cellular biochemistry Vol. 118; no. 10; pp. 3225 - 3236
• Main Authors: Li, Xiao, Xu, Dong‐Hui, Liu, Fan, Liu, Guo‐Yan, Lu, Kun, Deng, Xiao‐Ling, Li, Qi‐Fu, Shi, Song‐Lin
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.10.2017
• Subjects: Acylation; Adhesive strength; Biological activity; Cancer; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Cell Adhesion; Cell adhesion & migration; Cell growth; Cell Line, Tumor; Cell migration; Cell Movement; Cell Proliferation; CELLULAR LOCALIZATION; Cytoplasm; Hepatocellular carcinoma; Hepatocytes; Hepatoma; Humans; INTERACTIONAL PROTEINS; Liver; LIVER CANCER; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Neoplasm Proteins - metabolism; NPM; Nuclear Proteins - metabolism; Nucleoli; Protein Transport; Proteins; Relocation; Solid tumors; tRNA; Tumors; INTERACTIONAL PROTEINS; NPM; LIVER CANCER; CELLULAR LOCALIZATION
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.25971

ABSTRACT Nucleophosmin(NPM), heavily implicated in diverse solid tumors, is an important multifunctional protein mainly located in the nucleolus. Our previous study confirmed that NPM can also localize and accumulate in the cytoplasm of liver cancer cells. However, the role of cytoplasmic NPM (NPMc +) is unclear. Here, we showed that both nucleolar NPM and NPMc+ could promote cell proliferation, although the effect of NPMc+ was weaker than that of NPM. Cell adhesion ability of hepatoma cells was significantly reduced to a greater extent by NPMc+ expression. Nucleolar NPM enhanced cell migration and invasion, whereas NPMc+ impeded cell migration and invasion. The investigation of NPM interactional proteins by proteomic method demonstrated that the NPM was involved in multiple biological processes. By contrast, the interactional proteins of NPMc+ were mainly implicated in tRNA amino acylation regulation. The interactional network of NPMc+ was significantly small and simple. These results suggested that relocation of NPM altered its interactional network and consequently disturbed the primary functions, including cell proliferation, adhesion, migration, and invasion. NPM plays a promotional role in cancer and the reducing relocation may be a potential therapeutic target for hepatocellular carcinoma. J. Cell. Biochem. 118: 3225–3236, 2017. © 2017 Wiley Periodicals, Inc. NPM, a nucleolar phosphorylated protein heavily implicated in cancers of diverse origins, is an important multifunctional protein mainly located in the nucleolus. Our results suggested that relocalization altered the interactional network of NPM and consequently disturbed these primary functions, including cell proliferation, adhesion, migration, and invasion.