Cell‐free protein synthesis enables one‐pot cascade biotransformation in an aqueous‐organic biphasic system

• Published in: Biotechnology and bioengineering Vol. 117; no. 12; pp. 4001 - 4008
• Main Authors: Liu, Wan‐Qiu, Wu, Changzhu, Jewett, Michael C., Li, Jian
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.12.2020
• Subjects: biocatalysis; Biocatalysts; Biotransformation; biphasic system; Cascade chemical reactions; cascade reaction; Cell-Free System; cell‐free protein synthesis; Chemical reactions; Chemical synthesis; Conversion; Enzymes; Escherichia coli - chemistry; Escherichia coli - metabolism; Green Fluorescent Proteins - biosynthesis; Organic solvents; Protein Biosynthesis; Protein synthesis; Proteins; Solvents; Styrene; Substrates; biotransformation; cell-free protein synthesis; cascade reaction; biocatalysis; biphasic system

Biocatalytic cascade reactions have become increasingly important and useful for chemical synthesis. However, biocatalysts are often incompatible with organic solvents, which prohibits many cascade reactions involving nonpolar substrates. In this study, we used cell‐free protein synthesis (CFPS) to...