Distinction of mouse interferon-alpha subtypes by polymerase chain reaction utilizing consensus primers and type-specific oligonucleotide probes

• Published in: Journal of interferon research Vol. 14; no. 3; p. 117
• Main Authors: Hughes, Jr, T K, Chin, R, Tyring, S K, Rady, P L
• Format: Journal Article
• Language: English
• Published: United States 01.06.1994
• Subjects: Animals; Base Sequence; Cell Line; Consensus Sequence; DNA Primers; Interferon-alpha - analysis; Mice; Molecular Sequence Data; Oligonucleotide Probes; Polymerase Chain Reaction; RNA - isolation & purification

We have developed a novel method to study the subtype-specific expression of interferon-alpha (IFN-alpha) in the mouse system: we synthesized and used consensus oligonucleotide primers to allow simultaneous polymerase chain reaction (PCR) amplification of multiple murine IFN-alpha gene sequences. In...