Nuclear export signal in CDC25B
CDC25B is a dual-specificity phosphatase that activates CDK1/cyclin B. The nuclear exclusion of CDC25B is controlled by the binding of 14-3-3 to the nuclear export signal (NES) of CDC25B, which was reported to be amino acids H28 to L40 in the N-terminal region of CDC25B. In studying the subcellular...
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| Published in | Biochemical and biophysical research communications Vol. 316; no. 1; pp. 226 - 232 |
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| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
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United States
Elsevier Inc
26.03.2004
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| Abstract | CDC25B is a dual-specificity phosphatase that activates CDK1/cyclin B. The nuclear exclusion of CDC25B is controlled by the binding of 14-3-3 to the nuclear export signal (NES) of CDC25B, which was reported to be amino acids H28 to L40 in the N-terminal region of CDC25B. In studying the subcellular localization of CDC25B, we found a functional NES at V52 to L65, the sequence of which is
VTT
LTQT
MHD
LAG
L, where bold letters are leucine or hydrophobic amino acids frequently seen in an NES. The deletion of this NES sequence caused the mutant protein to locate exclusively in nuclei, while NES-fused GFP was detected in the cytoplasm. Moreover, the introduction of point mutations at some of the critical amino acids impaired cytoplasmic localization. Treatment with leptomycin B, a potent inhibitor of CRM1/exportin1, disrupted the cytoplasmic localization of both Flag-tagged CDC25B and NES-fused GFP. From these results, we concluded that the sequence we found is a bona fide NES of CDC25B. |
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| AbstractList | CDC25B is a dual-specificity phosphatase that activates CDK1/cyclin B. The nuclear exclusion of CDC25B is controlled by the binding of 14-3-3 to the nuclear export signal (NES) of CDC25B, which was reported to be amino acids H28 to L40 in the N-terminal region of CDC25B. In studying the subcellular localization of CDC25B, we found a functional NES at V52 to L65, the sequence of which is VTTLTQTMHDLAGL, where bold letters are leucine or hydrophobic amino acids frequently seen in an NES. The deletion of this NES sequence caused the mutant protein to locate exclusively in nuclei, while NES-fused GFP was detected in the cytoplasm. Moreover, the introduction of point mutations at some of the critical amino acids impaired cytoplasmic localization. Treatment with leptomycin B, a potent inhibitor of CRM1/exportin1, disrupted the cytoplasmic localization of both Flag-tagged CDC25B and NES-fused GFP. From these results, we concluded that the sequence we found is a bona fide NES of CDC25B. CDC25B is a dual-specificity phosphatase that activates CDK1/cyclin B. The nuclear exclusion of CDC25B is controlled by the binding of 14-3-3 to the nuclear export signal (NES) of CDC25B, which was reported to be amino acids H28 to L40 in the N-terminal region of CDC25B. In studying the subcellular localization of CDC25B, we found a functional NES at V52 to L65, the sequence of which is VTT LTQT MHD LAG L, where bold letters are leucine or hydrophobic amino acids frequently seen in an NES. The deletion of this NES sequence caused the mutant protein to locate exclusively in nuclei, while NES-fused GFP was detected in the cytoplasm. Moreover, the introduction of point mutations at some of the critical amino acids impaired cytoplasmic localization. Treatment with leptomycin B, a potent inhibitor of CRM1/exportin1, disrupted the cytoplasmic localization of both Flag-tagged CDC25B and NES-fused GFP. From these results, we concluded that the sequence we found is a bona fide NES of CDC25B. |
| Author | Nakagama, Hitoshi Uchida, Sanae Shimura, Mari Ishizaka, Yukihito Matsunaga, Tsukasa Ohtsubo, Motoaki Hirata, Masato Yoshida, Minoru Yamashita, Katsumi |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/15003534$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1016_j_ydbio_2008_02_028 crossref_primary_10_1158_0008_5472_CAN_09_0869 crossref_primary_10_1093_jb_mvj079 crossref_primary_10_1158_0008_5472_CAN_10_2453 crossref_primary_10_1089_cbr_2014_1701 crossref_primary_10_1016_j_bbamcr_2008_12_015 crossref_primary_10_1021_cr400585q crossref_primary_10_1242_jcs_01395 crossref_primary_10_1038_sj_onc_1209962 crossref_primary_10_1074_jbc_M112_367839 crossref_primary_10_1016_j_ceb_2006_02_003 |
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| Keywords | GFP 14-3-3 Subcellular localization CDC25B Nuclear export signal Leptomycin B |
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| SubjectTerms | 14-3-3 Active Transport, Cell Nucleus Amino Acid Sequence cdc25 Phosphatases - analysis cdc25 Phosphatases - chemistry cdc25 Phosphatases - genetics CDC25B Cell Cycle Proteins - analysis Cell Cycle Proteins - chemistry Cell Cycle Proteins - genetics Cell Nucleus - chemistry Cell Nucleus - metabolism Cytoplasm - chemistry Fatty Acids, Unsaturated - pharmacology GFP Humans Hydrophobic and Hydrophilic Interactions Leptomycin B Molecular Sequence Data Mutation Nuclear export signal Protein Sorting Signals Subcellular localization |
| Title | Nuclear export signal in CDC25B |
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