DNA-free directed assembly in single-molecule cut-and-paste

Single-molecule cut-and-paste facilitates bottom-up directed assembly of nanoscale biomolecular networks in defined geometries and enables analysis with spatio-temporal resolution. However, arrangement of diverse molecules of interest requires versatile handling systems. The novel DNA-free, genetica...

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Bibliographic Details
Published inNanoscale Vol. 11; no. 2; pp. 407 - 411
Main Authors Erlich, Katherine R, Sedlak, Steffen M, Jobst, Markus A, Milles, Lukas F, Gaub, Hermann E
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 03.01.2019
Subjects
Assembly
Deoxyribonucleic acid
DNA
Enzymes, Immobilized - chemistry
Enzymes, Immobilized - metabolism
Fluorescent Dyes
Immobilized Proteins - chemistry
Immobilized Proteins - metabolism
Microscopy, Atomic Force
Microscopy, Fluorescence
Models, Molecular
Nanostructures - chemistry
Nanostructures - ultrastructure
Nanotechnology - methods
Temporal resolution
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Summary:Single-molecule cut-and-paste facilitates bottom-up directed assembly of nanoscale biomolecular networks in defined geometries and enables analysis with spatio-temporal resolution. However, arrangement of diverse molecules of interest requires versatile handling systems. The novel DNA-free, genetically encodable scheme described here utilises an orthogonal handling strategy to promote arrangement of enzymes and enzyme networks.
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SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:2040-3364
2040-3372
DOI:10.1039/c8nr08636b