Denatured proteins facilitate the formation of the football-shaped GroEL-(GroES)2 complex

• Published in: Biochemical journal Vol. 427; no. 2; p. 247
• Main Authors: Sameshima, Tomoya, Iizuka, Ryo, Ueno, Taro, Funatsu, Takashi
• Format: Journal Article
• Language: English
• Published: England 15.04.2010
• Subjects: Adenosine Diphosphate - pharmacology; Adenosine Triphosphate - pharmacology; Chaperonin 10 - chemistry; Chaperonin 10 - metabolism; Chaperonin 60 - chemistry; Chaperonin 60 - metabolism; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - metabolism; Fluorescence Resonance Energy Transfer; Kinetics; Multiprotein Complexes - chemistry; Protein Binding; Protein Conformation; Protein Denaturation

Controversy exists over whether the chaperonin GroEL forms a GroEL-(GroES)2 complex (football-shaped complex) during its reaction cycle. We have revealed previously the existence of the football-shaped complex in the chaperonin reaction cycle using a FRET (fluorescence resonance energy transfer) ass...