Reactive oxidants mediate TNF-alpha-induced leukocyte adhesion to rat mesenteric venular endothelium

• Published in: The American journal of physiology Vol. 269; no. 6 Pt 2; p. H1833
• Main Authors: Morita, Y, Clemens, M G, Miller, L S, Rangan, U, Kondo, S, Miyasaka, M, Yoshikawa, T, Bulkley, G B
• Format: Journal Article
• Language: English
• Published: United States 01.12.1995
• Subjects: Allopurinol - pharmacology; Animals; Antibodies - pharmacology; Cell Adhesion; Endothelium, Vascular - physiology; Intercellular Adhesion Molecule-1 - immunology; Leukocytes - drug effects; Leukocytes - physiology; Male; Oxidoreductases - antagonists & inhibitors; Oxidoreductases - pharmacology; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species - metabolism; Splanchnic Circulation; Tumor Necrosis Factor-alpha - pharmacology; Venules - physiology

We investigated the role of reactive oxygen metabolites (ROMs) as potential mediators of tumor necrosis factor-alpha (TNF-alpha)-stimulated neutrophil adhesion to rat mesenteric venules in vivo, using intravital microscopy and fixed whole mount preparations of mesentery. Intraperitoneal injection of...