Inoculation treatments affect the migration and colonisation of rhizobia in alfalfa (Medicago sativa L.) plants
Purpose: The purposes of this study were to characterise the migration and the colonisation dynamics of two different fluorescent-tagged rhizobia in various alfalfa tissues (especially in seeds); and also to develop efficient inoculation treatments to promote colonisation ability of target rhizobia...
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Published in | Acta agriculturae Scandinavica. Section B, Soil and plant science Vol. 68; no. 3; pp. 199 - 212 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
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Taylor & Francis
03.04.2018
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Abstract | Purpose: The purposes of this study were to characterise the migration and the colonisation dynamics of two different fluorescent-tagged rhizobia in various alfalfa tissues (especially in seeds); and also to develop efficient inoculation treatments to promote colonisation ability of target rhizobia in elite seed varieties.
Materials and methods: Four treatments (root drench, root damaging and drench, root drench with matrine, and flower spray) were applied to inoculate alfalfa with two fluorescent-tagged rhizobia, Ensifer meliloti LZgn5f (gn5f) and Ensifer meliloti 12531f (12531f), at three different growth stages; bud, flower and pod stages. The migration and colonisation dynamics of the two fluorescent tagged rhizobia strains were monitored using UV lamp detection and a stereo fluorescence microscopy.
Results: The results showed that both rhizobia strains mainly colonised the roots and could migrate to aerial tissues. In aerial tissues, when alfalfa plants were inoculated during the bud stage, both rhizobia strains mainly colonised the leaves and stems; during the flower stage, a spray inoculation treatment resulted in more 12531f colonising reproductive tissues, while during the pod stage, more rhizobial strains gn5f colonised seeds using the root drench with matrine treatment.
Conclusions: These results indicate that endophytic rhizobia are natural inhabitants of internal regions of roots, stems, leaves and that the endophytes may arise from reproductive tissues, such as seeds. Understanding the population dynamics of endophytic rhizobia in alfalfa would considerably improve the survival of target rhizobia during seed transfer. Combining target endogenous rhizobial species with good alfalfa seed varieties may lead to the development of a novel breeding method. |
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AbstractList | Purpose: The purposes of this study were to characterise the migration and the colonisation dynamics of two different fluorescent-tagged rhizobia in various alfalfa tissues (especially in seeds); and also to develop efficient inoculation treatments to promote colonisation ability of target rhizobia in elite seed varieties. Materials and methods: Four treatments (root drench, root damaging and drench, root drench with matrine, and flower spray) were applied to inoculate alfalfa with two fluorescent-tagged rhizobia, Ensifer meliloti LZgn5f (gn5f) and Ensifer meliloti 12531f (12531f), at three different growth stages; bud, flower and pod stages. The migration and colonisation dynamics of the two fluorescent tagged rhizobia strains were monitored using UV lamp detection and a stereo fluorescence microscopy. Results: The results showed that both rhizobia strains mainly colonised the roots and could migrate to aerial tissues. In aerial tissues, when alfalfa plants were inoculated during the bud stage, both rhizobia strains mainly colonised the leaves and stems; during the flower stage, a spray inoculation treatment resulted in more 12531f colonising reproductive tissues, while during the pod stage, more rhizobial strains gn5f colonised seeds using the root drench with matrine treatment. Conclusions: These results indicate that endophytic rhizobia are natural inhabitants of internal regions of roots, stems, leaves and that the endophytes may arise from reproductive tissues, such as seeds. Understanding the population dynamics of endophytic rhizobia in alfalfa would considerably improve the survival of target rhizobia during seed transfer. Combining target endogenous rhizobial species with good alfalfa seed varieties may lead to the development of a novel breeding method. Purpose: The purposes of this study were to characterise the migration and the colonisation dynamics of two different fluorescent-tagged rhizobia in various alfalfa tissues (especially in seeds); and also to develop efficient inoculation treatments to promote colonisation ability of target rhizobia in elite seed varieties. Materials and methods: Four treatments (root drench, root damaging and drench, root drench with matrine, and flower spray) were applied to inoculate alfalfa with two fluorescent-tagged rhizobia, Ensifer meliloti LZgn5f (gn5f) and Ensifer meliloti 12531f (12531f), at three different growth stages; bud, flower and pod stages. The migration and colonisation dynamics of the two fluorescent tagged rhizobia strains were monitored using UV lamp detection and a stereo fluorescence microscopy. Results: The results showed that both rhizobia strains mainly colonised the roots and could migrate to aerial tissues. In aerial tissues, when alfalfa plants were inoculated during the bud stage, both rhizobia strains mainly colonised the leaves and stems; during the flower stage, a spray inoculation treatment resulted in more 12531f colonising reproductive tissues, while during the pod stage, more rhizobial strains gn5f colonised seeds using the root drench with matrine treatment. Conclusions: These results indicate that endophytic rhizobia are natural inhabitants of internal regions of roots, stems, leaves and that the endophytes may arise from reproductive tissues, such as seeds. Understanding the population dynamics of endophytic rhizobia in alfalfa would considerably improve the survival of target rhizobia during seed transfer. Combining target endogenous rhizobial species with good alfalfa seed varieties may lead to the development of a novel breeding method. |
Author | Miao, Yang-yang Kang, Wen-juan Shi, Shang-li Nie, Zhong-nan Frazier, Taylor P |
Author_xml | – sequence: 1 givenname: Yang-yang surname: Miao fullname: Miao, Yang-yang organization: College of Grassland Science, Key Laboratory for Grassland Ecosystem of Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Centers for Grazing Land Ecosystem Sustainability, Gansu Agricultural University – sequence: 2 givenname: Shang-li orcidid: 0000-0002-3304-8030 surname: Shi fullname: Shi, Shang-li email: 2470398455@qq.com organization: College of Grassland Science, Key Laboratory for Grassland Ecosystem of Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Centers for Grazing Land Ecosystem Sustainability, Gansu Agricultural University – sequence: 3 givenname: Zhong-nan orcidid: 0000-0001-7670-1581 surname: Nie fullname: Nie, Zhong-nan organization: Department of Economic Development, Jobs, Transport and Resources, Private Bag 105 – sequence: 4 givenname: Wen-juan surname: Kang fullname: Kang, Wen-juan organization: College of Grassland Science, Key Laboratory for Grassland Ecosystem of Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Centers for Grazing Land Ecosystem Sustainability, Gansu Agricultural University – sequence: 5 givenname: Taylor P surname: Frazier fullname: Frazier, Taylor P organization: Department of Plant Sciences, University of Tennessee |
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SubjectTerms | Alfalfa Breeding methods colonisation dynamics Colonization Endophytes Fluorescence Fluorescence microscopy fluorescent tagged rhizobia Inhabitants Inoculation inoculation treatments Leaves Migration Plant breeding Plant tissues reproductive stage Roots Seeds Stems |
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Title | Inoculation treatments affect the migration and colonisation of rhizobia in alfalfa (Medicago sativa L.) plants |
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