Development of an immunomagnetic nanoparticle-based lateral flow assay for detecting major kiwi allergen Act d1 in processed foods

• Published in: European food research & technology Vol. 248; no. 6; pp. 1601 - 1613
• Main Authors: Tsai, Wen-Che, Yin, Hsin-Yi, Leong, Weng-Tong, Tseng, Cheng-Yen, Wen, Hsiao-Wei
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2022; Springer Nature B.V
• Subjects: Agriculture; allergenicity; Allergens; Allergies; Analytical Chemistry; Assaying; Biotechnology; Chemistry; Chemistry and Materials Science; Coefficient of variation; detection limit; Food; Food allergies; Food processing; food research; Food Science; Forestry; fruits; hypersensitivity; Immunomagnetic separation; juices; Nanoparticles; Nutrients; Original Paper; Processed foods; Lateral flow assay; Act d1; Immunomagnetic nanoparticles; Kiwi allergy

Kiwi is famous for its health-giving nutrients, but it is also a well-known allergenic fruit. In this work, an immunomagnetic nanoparticle-based lateral flow assay (IMNP-based LFA) is developed for detecting the major kiwi allergen, Act d1. Immunomagnetic separation by IMNPs is used to reduce the ma...