GDNF Stimulates Rod Photoreceptors and Dopaminergic Amacrine Cells in Chicken Retinal Reaggregates

• Published in: Investigative ophthalmology & visual science Vol. 48; no. 11; pp. 5306 - 5314
• Main Authors: Volpert, Katja N, Rothermel, Andree, Layer, Paul G
• Format: Journal Article
• Language: English
• Published: Rockville, MD ARVO 01.11.2007; Association for Research in Vision and Ophtalmology
• Subjects: Amacrine Cells - drug effects; Amacrine Cells - metabolism; Animals; Antisense Elements (Genetics); Biological and medical sciences; Blotting, Western; Cell Count; Chick Embryo; Down-Regulation; Eye and associated structures. Visual pathways and centers. Vision; Fluorescent Antibody Technique, Indirect; Fundamental and applied biological sciences. Psychology; Gene Expression; Glial Cell Line-Derived Neurotrophic Factor - genetics; Glial Cell Line-Derived Neurotrophic Factor - pharmacology; Oligonucleotides, Antisense; Receptors, Dopamine - metabolism; Retinal Rod Photoreceptor Cells - drug effects; Retinal Rod Photoreceptor Cells - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Rhodopsin - biosynthesis; Rhodopsin - genetics; RNA, Messenger - metabolism; Spheroids, Cellular - metabolism; Transfection; Tyrosine 3-Monooxygenase - biosynthesis; Tyrosine 3-Monooxygenase - genetics; Vertebrates: nervous system and sense organs; Vertebrata; Rod; Photoreceptor; Retina; Chicken; Ophthalmology; Aves; Cell; Retinal

To investigate the role(s) of glial cell line-derived neurotrophic factor (GDNF) on expression of rod photoreceptor and dopaminergic amacrine cell-specific genes in an in vitro reaggregate model of the chick retina. Retinal reaggregates derived from embryonic day (E)6 chicks (rosetted spheroids) wer...