Determination of urea concentration using urease-containing polyelectrolyte microcapsules

• Published in: Analytical methods Vol. 11; no. 12; pp. 1585 - 159
• Main Authors: Kim, Aleksandr L, Musin, Egor V, Dubrovskii, Alexey V, Tikhonenko, Sergey A
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 21.03.2019
• Subjects: adsorption; analytical methods; Blood levels; Coefficient of variation; Deviation; Diagnostic systems; diagnostic techniques; electrolytes; encapsulation; Enzymes; Enzymology; Linearity; Microcapsules; Microencapsulation; Polyelectrolytes; proteinases; Proteolysis; Proteolytic enzymes; Solid surfaces; Urea; Urease; Urine

Nowadays, the enzymological method used in clinical diagnostics has several problems: a short lifetime of the enzyme sensor in solution, its susceptibility in the presence of proteolytic enzymes, and the impossibility of multiple uses. We proposed the modification of the standard method of urea dete...