BsuMI regulates DNA transformation in Bacillus subtilis besides the defense system and the constructed strain with BsuMI-absence is applicable as a universal transformation platform for wild-type Bacillus

• Published in: Microbial cell factories Vol. 23; no. 1; pp. 225 - 15
• Main Authors: Xingya, Zhao, Xiaoping, Fu, Jie, Zhen, Jun, Yang, Hongchen, Zheng, Wenqin, Bai, Hui, Song
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 09.08.2024; BMC
• Subjects: Analysis; Bacillus (Bacteria); Bacillus species; Bacillus subtilis - genetics; Bacillus subtilis - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Cellular signal transduction; Control; Cytochrome oxidase; DNA Transformation Competence; Genetic aspects; Identification and classification; Metabolites; Permeability; Plasmid transformation; Plasmids - genetics; Plasmids - metabolism; Regulatory mechanism; Restriction-modification system; Transformation, Bacterial; China; Plasmid transformation; Bacillus species; Restriction-modification system; Regulatory mechanism

To effectively introduce plasmids into Bacillus species and conduct genetic manipulations in Bacillus chassis strains, it is essential to optimize transformation methods. These methods aim to extend the period of competence and enhance the permeability of the cell membrane to facilitate the entry of...