Validation of Acoustic Wave Induced Traumatic Brain Injury in Rats

• Published in: Brain sciences Vol. 7; no. 6; p. 59
• Main Authors: Berman, Sean, Uhlendorf, Toni L, Mills, David K, Lander, Elliot B, Berman, Mark H, Cohen, Randy W
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02.06.2017; MDPI
• Subjects: Acoustics; Cortex (frontal); Histology; Motor ability; Motor skill; Rodents; Traumatic brain injury; traumatic brain injury (TBI); concussion; acoustic shockwave TBI; mild TBI; closed-head TBI
• ISSN: 2076-3425; 2076-3425
• DOI: 10.3390/brainsci7060059

This study looked to validate the acoustic wave technology of the Storz-D-Actor that inflicted a consistent closed-head, traumatic brain injury (TBI) in rats. We studied a range of single pulse pressures administered to the rats and observed the resulting decline in motor skills and memory. Histology was observed to measure and confirm the injury insult. Four different acoustic wave pressures were studied using a single pulse: 0, 3.4, 4.2 and 5.0 bar ( = 10 rats per treatment group). The pulse was administered to the left frontal cortex. Rotarod tests were used to monitor the rats' motor skills while the water maze test was used to monitor memory deficits. The rats were then sacrificed ten days post-treatment for histological analysis of TBI infarct size. The behavioral tests showed that acoustic wave technology administered an effective insult causing significant decreases in motor abilities and memory. Histology showed dose-dependent damage to the cortex infarct areas only. This study illustrates that the Storz D-Actor effectively induces a repeatable TBI infarct, avoiding the invasive procedure of a craniotomy often used in TBI research.