Characterization of the cytokine network at a single cell level in mice with collagen-induced arthritis using a dual color ELISPOT assay

• Published in: Journal of interferon & cytokine research Vol. 20; no. 1; pp. 55 - 61
• Main Authors: Okamoto, Y, Gotoh, Y, Tokui, H, Mizuno, A, Kobayashi, Y, Nishida, M
• Format: Journal Article
• Language: English
• Published: United States Mary Ann Liebert, Inc 01.01.2000
• Subjects: Animals; Autoimmune Diseases - chemically induced; Autoimmune Diseases - immunology; Autoimmune Diseases - metabolism; Cattle; Collagen - immunology; Collagen - toxicity; Collagen Diseases - chemically induced; Collagen Diseases - immunology; Collagen Diseases - metabolism; Color; Disease Progression; Enzyme-Linked Immunosorbent Assay - methods; g-Interferon; Immunization; Interleukin-12 - secretion; Interleukin-2 - secretion; Interleukin-4 - secretion; Lymph Nodes - immunology; Lymph Nodes - pathology; Male; Mice; Mice, Inbred DBA; Organ Specificity; Peritoneal Cavity - cytology; Spleen - immunology; Spleen - pathology; Th1 Cells - immunology; Th1 Cells - secretion; Th2 Cells - immunology

Collagen-induced arthritis (CIA) in mice has been classified as a Thl-mediated disease. However, most evidence for this has been obtained by indirect experiments; for example, the administration of neutralizing anti-interferon-gamma (IFN-gamma) antibody reduced the severity of arthritis. To obtain d...