Tandem fluorescence and Raman (fluoRaman) characterisation of a novel photosensitiser in colorectal cancer cell line SW480

• Published in: Analyst (London) Vol. 143; no. 24; pp. 6113 - 6120
• Main Authors: Gala de Pablo, Julia, Chisholm, David R, Steffen, Andreas, Nelson, Amanda K, Mahler, Christoph, Marder, Todd B, Peyman, Sally A, Girkin, John M, Ambler, Carrie A, Whiting, Andrew, Evans, Stephen D
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 03.12.2018
• Subjects: Biological activity; Cancer; Cell Line, Tumor; Chemical compounds; Cinnamates - chemical synthesis; Cinnamates - chemistry; Cinnamates - metabolism; Colorectal cancer; Fluorescence; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Fluorescent Dyes - metabolism; Functional groups; Humans; Imaging; Lipids; Microscopy; Microscopy, Confocal - methods; Photosensitizing Agents - chemical synthesis; Photosensitizing Agents - chemistry; Photosensitizing Agents - metabolism; Polar environments; Quinolines - chemical synthesis; Quinolines - chemistry; Quinolines - metabolism; Spectrometry, Fluorescence - methods; Spectrum Analysis, Raman - methods

The development of new imaging tools, molecules and modalities is crucial to understanding biological processes and the localised cellular impact of bioactive compounds. A small molecule photosensitiser, DC473, has been designed to be both highly fluorescent and to exhibit a strong Raman signal in t...