Tandem fluorescence and Raman (fluoRaman) characterisation of a novel photosensitiser in colorectal cancer cell line SW480

• Published in: Analyst (London) Vol. 143; no. 24; pp. 6113 - 6120
• Main Authors: Gala de Pablo, Julia, Chisholm, David R, Steffen, Andreas, Nelson, Amanda K, Mahler, Christoph, Marder, Todd B, Peyman, Sally A, Girkin, John M, Ambler, Carrie A, Whiting, Andrew, Evans, Stephen D
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 03.12.2018
• Subjects: Biological activity; Cancer; Cell Line, Tumor; Chemical compounds; Cinnamates - chemical synthesis; Cinnamates - chemistry; Cinnamates - metabolism; Colorectal cancer; Fluorescence; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Fluorescent Dyes - metabolism; Functional groups; Humans; Imaging; Lipids; Microscopy; Microscopy, Confocal - methods; Photosensitizing Agents - chemical synthesis; Photosensitizing Agents - chemistry; Photosensitizing Agents - metabolism; Polar environments; Quinolines - chemical synthesis; Quinolines - chemistry; Quinolines - metabolism; Spectrometry, Fluorescence - methods; Spectrum Analysis, Raman - methods
• DOI: 10.1039/c8an01461b
• ISSN: 0003-2654

The development of new imaging tools, molecules and modalities is crucial to understanding biological processes and the localised cellular impact of bioactive compounds. A small molecule photosensitiser, DC473, has been designed to be both highly fluorescent and to exhibit a strong Raman signal in the cell-silent region of the Raman spectrum due to a diphenylacetylene structure. DC473 has been utilised to perform a range of novel tandem fluorescence and Raman (fluoRaman) imaging experiments, enabling a thorough examination of the compound's cellular localisation, exemplified in colorectal cancer cells (SW480). This multifunctional fluoRaman imaging modality revealed the presence of the compound in lipid droplets and only a weak signal in the cytosol, by both Raman and fluorescence imaging. In addition, Raman microscopy detected the compound in a cell compartment we labelled as the nucleolus, whereas fluorescence microscopy did not detect the fluoRaman probe due to solvatochromatic effects in a local polar environment. This last finding was only possible with the use of tandem confocal Raman and fluorescence methods. By following the approach detailed herein, incorporation of strong Raman functional groups into fluorophores can enable a plethora of fluoRaman experiments, shedding further light on potential drug compound's cellular behaviour and biological activity.