Molecular Staging of Prostate Cancer: Comparison of Nested Reverse Transcription Polymerase Chain Reaction Assay Using Prostate Specific Antigen Versus Prostate Specific Membrane Antigen as Primer

• Published in: International journal of urology Vol. 5; no. 4; pp. 349 - 356
• Main Authors: Okegawa, Takatsugu, Yoshioka, Junichi, Morita, Ryoji, Nutahara, Kikuo, Tsukada, Yutaka, Higashihara, Eiji
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.07.1998
• Subjects: Aged; Antigens, Surface; Carboxypeptidases - genetics; DNA Primers; DNA, Neoplasm - analysis; Female; Glutamate Carboxypeptidase II; Humans; K562 Cells - physiology; Male; Middle Aged; Neoplasm Staging; Physical Examination; polymerase chain reaction; Polymerase Chain Reaction - methods; Predictive Value of Tests; prostate; prostate specific antigen; prostate specific membrane antigen; Prostate-Specific Antigen - genetics; Prostatic Hyperplasia - genetics; Prostatic Hyperplasia - pathology; Prostatic Neoplasms - genetics; Prostatic Neoplasms - pathology; Sensitivity and Specificity; Sequence Analysis, DNA

Background: We examined the utility for prostate cancer staging of nested reverse transcription polymerase chain reaction (RT‐PCR) using either prostate specific antigen (PSA) or prostate specific membrane antigen (PSM) as primer. Methods: LNCaP cells were used for the in vitro quantification of RT‐...