Molecular Staging of Prostate Cancer: Comparison of Nested Reverse Transcription Polymerase Chain Reaction Assay Using Prostate Specific Antigen Versus Prostate Specific Membrane Antigen as Primer
Background: We examined the utility for prostate cancer staging of nested reverse transcription polymerase chain reaction (RT‐PCR) using either prostate specific antigen (PSA) or prostate specific membrane antigen (PSM) as primer. Methods: LNCaP cells were used for the in vitro quantification of RT‐...
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Published in | International journal of urology Vol. 5; no. 4; pp. 349 - 356 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.07.1998
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Subjects | |
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Abstract | Background: We examined the utility for prostate cancer staging of nested reverse transcription polymerase chain reaction (RT‐PCR) using either prostate specific antigen (PSA) or prostate specific membrane antigen (PSM) as primer.
Methods: LNCaP cells were used for the in vitro quantification of RT‐PCR. RT‐PCR was performed on the peripheral blood of 105 control subjects and 63 patients with prostate cancer (32 who eventually underwent radical prostatectomy and 31 with clinical stage D2 cancer).
Results: The nested RT‐PCR for the PSA and PSM primers was able to detect 1 LNCaP cell per 106 leukemia (K562) cells. Noneof the control subjects was found positive for the presence of prostate cancer cells by nested RT‐PCR. In the 32‐patient surgery group, the results of nested RT‐PCR were significantly correlated with the pathologic stage of the cancer when using PSM primers (P=2.00times10‐3 by Kendall's correlation test) but not when using PSA primers (P=0.06). Extraprostatic extension was significantly more closely correlated with positive PSM nested RT‐PCR results (P=0.012 by Fisher's exact probability test) than with positive results of PSA, nested RT‐PCR, digital rectal examination, CT imaging, level of serum PSA or Cleason score. In the untreated stage D2 patients, the positive result rate of PSM nested RT‐PCR was significantly higher than that of PSA nested RT‐PCR (P=0.025 by McNemar test).
Conclusion: Nested RT‐PCR using PSM primers appears to predict the prostate cancer stage more accurately than does nested RT‐PCR using PSA primers or conventional clinical staging modalities. |
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AbstractList | We examined the utility for prostate cancer staging of nested reverse transcription polymerase chain reaction (RT-PCR) using either prostate specific antigen (PSA) or prostate specific membrane antigen (PSM) as primer.
LNCaP cells were used for the in vitro quantification of RT-PCR. RT-PCR was performed on the peripheral blood of 105 control subjects and 63 patients with prostate cancer (32 who eventually underwent radical prostatectomy and 31 with clinical stage D2 cancer).
The nested RT-PCR for the PSA and PSM primers was able to detect 1 LNCaP cell per 10(6) leukemia (K562) cells. None of the control subjects was found positive for the presence of prostate cancer cells by nested RT-PCR. In the 32-patient surgery group, the results of nested RT-PCR were significantly correlated with the pathologic stage of the cancer when using PSM primers (P=2.00 x 10(-3) by Kendall's correlation test) but not when using PSA primers (P=0.06). Extraprostatic extension was significantly more closely correlated with positive PSM nested RT-PCR results (P=0.012 by Fisher's exact probability test) than with positive results of PSA, nested RT-PCR, digital rectal examination, CT imaging, level of serum PSA or Gleason score. In the untreated stage D2 patients, the positive result rate of PSM nested RT-PCR was significantly higher than that of PSA nested RT-PCR (P=0.025 by McNemar test).
Nested RT-PCR using PSM primers appears to predict the prostate cancer stage more accurately than does nested RT-PCR using PSA primers or conventional clinical staging modalities. Background: We examined the utility for prostate cancer staging of nested reverse transcription polymerase chain reaction (RT‐PCR) using either prostate specific antigen (PSA) or prostate specific membrane antigen (PSM) as primer. Methods: LNCaP cells were used for the in vitro quantification of RT‐PCR. RT‐PCR was performed on the peripheral blood of 105 control subjects and 63 patients with prostate cancer (32 who eventually underwent radical prostatectomy and 31 with clinical stage D2 cancer). Results: The nested RT‐PCR for the PSA and PSM primers was able to detect 1 LNCaP cell per 106 leukemia (K562) cells. Noneof the control subjects was found positive for the presence of prostate cancer cells by nested RT‐PCR. In the 32‐patient surgery group, the results of nested RT‐PCR were significantly correlated with the pathologic stage of the cancer when using PSM primers (P=2.00times10‐3 by Kendall's correlation test) but not when using PSA primers (P=0.06). Extraprostatic extension was significantly more closely correlated with positive PSM nested RT‐PCR results (P=0.012 by Fisher's exact probability test) than with positive results of PSA, nested RT‐PCR, digital rectal examination, CT imaging, level of serum PSA or Cleason score. In the untreated stage D2 patients, the positive result rate of PSM nested RT‐PCR was significantly higher than that of PSA nested RT‐PCR (P=0.025 by McNemar test). Conclusion: Nested RT‐PCR using PSM primers appears to predict the prostate cancer stage more accurately than does nested RT‐PCR using PSA primers or conventional clinical staging modalities. BACKGROUNDWe examined the utility for prostate cancer staging of nested reverse transcription polymerase chain reaction (RT-PCR) using either prostate specific antigen (PSA) or prostate specific membrane antigen (PSM) as primer.METHODSLNCaP cells were used for the in vitro quantification of RT-PCR. RT-PCR was performed on the peripheral blood of 105 control subjects and 63 patients with prostate cancer (32 who eventually underwent radical prostatectomy and 31 with clinical stage D2 cancer).RESULTSThe nested RT-PCR for the PSA and PSM primers was able to detect 1 LNCaP cell per 10(6) leukemia (K562) cells. None of the control subjects was found positive for the presence of prostate cancer cells by nested RT-PCR. In the 32-patient surgery group, the results of nested RT-PCR were significantly correlated with the pathologic stage of the cancer when using PSM primers (P=2.00 x 10(-3) by Kendall's correlation test) but not when using PSA primers (P=0.06). Extraprostatic extension was significantly more closely correlated with positive PSM nested RT-PCR results (P=0.012 by Fisher's exact probability test) than with positive results of PSA, nested RT-PCR, digital rectal examination, CT imaging, level of serum PSA or Gleason score. In the untreated stage D2 patients, the positive result rate of PSM nested RT-PCR was significantly higher than that of PSA nested RT-PCR (P=0.025 by McNemar test).CONCLUSIONNested RT-PCR using PSM primers appears to predict the prostate cancer stage more accurately than does nested RT-PCR using PSA primers or conventional clinical staging modalities. Background : We examined the utility for prostate cancer staging of nested reverse transcription polymerase chain reaction (RT‐PCR) using either prostate specific antigen (PSA) or prostate specific membrane antigen (PSM) as primer. Methods : LNCaP cells were used for the in vitro quantification of RT‐PCR. RT‐PCR was performed on the peripheral blood of 105 control subjects and 63 patients with prostate cancer (32 who eventually underwent radical prostatectomy and 31 with clinical stage D2 cancer). Results : The nested RT‐PCR for the PSA and PSM primers was able to detect 1 LNCaP cell per 10 6 leukemia (K562) cells. Noneof the control subjects was found positive for the presence of prostate cancer cells by nested RT‐PCR. In the 32‐patient surgery group, the results of nested RT‐PCR were significantly correlated with the pathologic stage of the cancer when using PSM primers ( P =2.00times10 ‐3 by Kendall's correlation test) but not when using PSA primers ( P =0.06). Extraprostatic extension was significantly more closely correlated with positive PSM nested RT‐PCR results ( P =0.012 by Fisher's exact probability test) than with positive results of PSA, nested RT‐PCR, digital rectal examination, CT imaging, level of serum PSA or Cleason score. In the untreated stage D2 patients, the positive result rate of PSM nested RT‐PCR was significantly higher than that of PSA nested RT‐PCR ( P =0.025 by McNemar test). Conclusion : Nested RT‐PCR using PSM primers appears to predict the prostate cancer stage more accurately than does nested RT‐PCR using PSA primers or conventional clinical staging modalities. |
Author | Yoshioka, Junichi Morita, Ryoji Nutahara, Kikuo Tsukada, Yutaka Okegawa, Takatsugu Higashihara, Eiji |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/9712443$$D View this record in MEDLINE/PubMed |
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Snippet | Background: We examined the utility for prostate cancer staging of nested reverse transcription polymerase chain reaction (RT‐PCR) using either prostate... We examined the utility for prostate cancer staging of nested reverse transcription polymerase chain reaction (RT-PCR) using either prostate specific antigen... Background : We examined the utility for prostate cancer staging of nested reverse transcription polymerase chain reaction (RT‐PCR) using either prostate... BACKGROUNDWe examined the utility for prostate cancer staging of nested reverse transcription polymerase chain reaction (RT-PCR) using either prostate specific... |
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SubjectTerms | Aged Antigens, Surface Carboxypeptidases - genetics DNA Primers DNA, Neoplasm - analysis Female Glutamate Carboxypeptidase II Humans K562 Cells - physiology Male Middle Aged Neoplasm Staging Physical Examination polymerase chain reaction Polymerase Chain Reaction - methods Predictive Value of Tests prostate prostate specific antigen prostate specific membrane antigen Prostate-Specific Antigen - genetics Prostatic Hyperplasia - genetics Prostatic Hyperplasia - pathology Prostatic Neoplasms - genetics Prostatic Neoplasms - pathology Sensitivity and Specificity Sequence Analysis, DNA |
Title | Molecular Staging of Prostate Cancer: Comparison of Nested Reverse Transcription Polymerase Chain Reaction Assay Using Prostate Specific Antigen Versus Prostate Specific Membrane Antigen as Primer |
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