Atmospheric pressure chemical ionization mass spectrometry and in-source fragmentation of lutein esters

• Published in: Journal of mass spectrometry. Vol. 38; no. 9; pp. 990 - 995
• Main Authors: Tian, Qingguo, Duncan, Christine J. G., Schwartz, Steven J.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.09.2003
• Subjects: Atmospheric Pressure; atmospheric pressure chemical ionization; Esters - analysis; Esters - chemistry; Flowers - chemistry; in-source collisionally induced dissociation; liquid chromatography/mass spectrometry; Lutein - chemistry; lutein diester; marigold; Plant Extracts - chemistry; Spectrometry, Mass, Electrospray Ionization - methods
• ISSN: 1076-5174; 1096-9888
• DOI: 10.1002/jms.514

Negative‐ion atmospheric pressure chemical ionization (APCI) mass spectrometry and in‐source collisionally induced dissociation (CID) were employed to obtain structural information of lutein esters from marigold extract. Both molecular ions and structurally significant fragments corresponding to the loss of fatty acids were observed in high abundance in the current study. Six lutein diesters including lauroylmyristoyl‐lutein (LML), dimyristoyl‐lutein (dML), myristoylpalmitoyl‐lutein (MPL), dipalmitoyl‐lutein (dPL), palmitoylstearoyl‐lutein (PSL) and distearoyl‐lutein (dSL) were characterized in a marigold flower extract. Breakdown curves (plots of relative ion abundance vs. internal energy) of three lutein diesters were established by monitoring the relative ion abundance of molecular and fragment ions at different cone voltages during negative‐ion APCI‐LC/MS analysis. Copyright © 2003 John Wiley & Sons, Ltd.