Label-free detection of miRNA cancer markers based on terminal deoxynucleotidyl transferase-induced copper nanoclusters

• Published in: Analytical biochemistry Vol. 585; p. 113346
• Main Authors: Li, Yiting, Tang, Dihong, Zhu, Li, Cai, Jingting, Chu, Chaonan, Wang, Jing, Xia, Man, Cao, Zhenzhen, Zhu, Hong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.11.2019
• Subjects: Cancer diagnosis; Copper nanoclusters; Label-free; miRNA-21; Terminal deoxynucleotidyl transferase; Copper nanoclusters; Label-free; Cancer diagnosis; Terminal deoxynucleotidyl transferase; miRNA-21

The variations in microRNA (miRNA) expression levels can be useful biomarkers for the diagnosis of different cancers. In this work, a label-free and sensitive fluorescent method for detection of miRNA-21 is described based on duplex-specific nuclease (DSN) assist target recycling and terminal deoxyn...