Poly(ethylene glycol) (PEG) microwells in microfluidics: Fabrication methods and applications

Immobilization of bioanalytes (e.g., protein, lipid membrane, and cells) within a microfludic channel is a useful strategy in diverse biological analysis including biosensor, diagnosis, and biochemical reactions. Such microfluidic systems can offer miniaturized platforms with distinct advantages suc...

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Published inBiochip journal Vol. 8; no. 4; pp. 241 - 253
Main Authors Kang, Do Hyun, Kim, Hong Nam, Kim, Pilnam, Suh, Kahp-Yang
Format Journal Article
LanguageEnglish
Published Heidelberg The Korean BioChip Society (KBCS) 01.12.2014
Springer Nature B.V
한국바이오칩학회
Subjects
Biomedical Engineering and Bioengineering
Biosensors
Biotechnology
Chemistry
Chemistry and Materials Science
Immobilization
Lipids
Membrane proteins
Microchannels
Microfluidic devices
Microfluidics
Microreactors
Platforms
Polydimethylsiloxane
Polyethylene glycol
Reagents
Review Article
Substrates
Tissue engineering
생물공학
Soft lithography
Microchannel
Poly(ethylene glycol) (PEG)
Microwell
Bioanalytes arrays
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ISSN1976-0280
2092-7843
DOI10.1007/s13206-014-8401-y

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Summary:Immobilization of bioanalytes (e.g., protein, lipid membrane, and cells) within a microfludic channel is a useful strategy in diverse biological analysis including biosensor, diagnosis, and biochemical reactions. Such microfluidic systems can offer miniaturized platforms with distinct advantages such as reduced use of samples or reagents and increased resolution. In particular, polymeric microfluidic devices (e.g., polydimethylsiloxane (PDMS)) bound to a substrate have been widely used. However the non-specific adsorption of bioanalytes is serious problem for microfluidic biological assays, especially for dilute samples. To overcome such limitations, a simple and widely applicable microfluidic channels combined with polyethylene glycol (PEG) microwells have been developed. In this review, we summarize the methods and application of PEG microwells with a particular emphasis on integrated microfluidic systems. The assembly between PEG microwell and microchannel enables precise delivery and manipulation of the biosamples, leading to development of miniaturized diagnostic assays, microreactors, and multiple screening platforms for tissue engineering and cell biology.
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G704-SER000001574.2014.8.4.004
ISSN:1976-0280
2092-7843
DOI:10.1007/s13206-014-8401-y