Long term kinetic measurements revealing precision and general performance of surface plasmon resonance biosensors

• Published in: Analytical biochemistry Vol. 530; pp. 94 - 103
• Main Authors: Steinicke, Franziska, Oltmann-Norden, Imke, Wätzig, Hermann
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2017
• Subjects: Antibodies, Monoclonal - immunology; Antigen-Antibody Reactions - physiology; beta 2-Microglobulin - analysis; beta 2-Microglobulin - immunology; Biosensing Techniques - methods; Biosensors; Control charts; Human β2 microglobulin; Humans; Image Processing, Computer-Assisted - methods; Kinetic constants; Kinetics; Surface plasmon resonance; Surface Plasmon Resonance - methods; Surface Properties; System performance; RU; IFC; SD; SE; System performance; SPR; ka; Control charts; KD; b2m; HPLC; CE; RSD; Kinetic constants; kt; GMP; ICH; EU; Human β2 microglobulin; USP; MCK; Rmax; SOP; RI; ITC; Surface plasmon resonance; SCK; Biosensors

This work presents an extensive parameter list that facilitates a survey of biosensor performance using Biacore instruments for kinetic binding studies. Six long term measurements were performed using a strongly interacting antigen-antibody (β2 microglobulin) system. Both Single Cycle Kinetic (SCK)...