Easy method for discriminating the origins of manila clam Ruditapes philippinarum with a dual-labelled PNA-probe-based melting curve analysis

• Published in: Biochip journal Vol. 9; no. 3; pp. 247 - 258
• Main Authors: Kim, Eun-Mi, Song, Min Sik, Hur, Deok Hwe, An, Cheul Min, Kang, Jung-Ha, Park, Jung Youn
• Format: Journal Article
• Language: English
• Published: Seoul The Korean BioChip Society (KBCS) 01.09.2015; Springer Nature B.V; 한국바이오칩학회
• Subjects: Binding; Biomedical Engineering and Bioengineering; Biotechnology; Chemistry; Chemistry and Materials Science; DNA probes; Fluorescence; Genotyping; Melt temperature; Melting; Melting curve; Nucleic acids; Original Article; Origins; Peptide nucleic acids; Probes; Ruditapes philippinarum; Thermal denaturation; 생물공학; Manila clam; PNA; Discrimination; MeltingArray; Real-time PCR

Probe-based fluorescence melting curve analysis is a powerful tool used to detect mutations, based on the melting temperature from the thermal denaturation of the probe-target hybrid. PNA (Peptide nucleic acid) probes have several advantages: easy to design and modify and do not give false negative...