Targeted Base Editing via RNA-Guided Cytidine Deaminases in Xenopus laevis Embryos

• Published in: Molecules and cells Vol. 40; no. 11; pp. 823 - 827
• Main Authors: Park, Dong-Seok, Yoon, Mijung, Kweon, Jiyeon, Jang, An-Hee, Kim, Yongsub, Choi, Sun-Cheol
• Format: Journal Article
• Language: English
• Published: United States Korean Society for Molecular and Cellular Biology 30.11.2017; 한국분자세포생물학회
• Subjects: Albinism, Oculocutaneous - genetics; Amino Acid Substitution; Animals; Cytidine Deaminase - metabolism; Gene Editing - methods; Monophenol Monooxygenase - genetics; Mutation Rate; Phenotype; Point Mutation; Rapid Report; RNA, Guide, CRISPR-Cas Systems - genetics; Xenopus laevis - embryology; Xenopus laevis - genetics; Xenopus Proteins - genetics; 생물학; CRISPR/Cas9; base editing; Xenopus laevis; genome engineering

Genome editing using programmable nucleases such as CRISPR/Cas9 or Cpf1 has emerged as powerful tools for gene knock-out or knock-in in various organisms. While most genetic diseases are caused by point mutations, these genome-editing approaches are inefficient in inducing single-nucleotide substitu...