Evaluation of a feline-specific multiplex, bead-based assay for detection of cytokines, chemokines, growth factors, and other immunologically active proteins in serum and plasma samples from cats

• Published in: American journal of veterinary research Vol. 77; no. 5; pp. 495 - 504
• Main Authors: Halpin, Rachel E., Saunders, Rebecca S., Thompson, Beverly J., Rohde Newgent, Allison S., Amorim, Juliana, Melillo, Gabrielle N., DeClue, Amy E.
• Format: Journal Article
• Language: English
• Published: United States American Veterinary Medical Association 01.05.2016
• Subjects: adverse effects; Animals; blood proteins; blood sampling; blood serum; cats; Cats - blood; chemokines; Chemokines - blood; Cytokines - blood; Enzyme-Linked Immunosorbent Assay - veterinary; Female; growth factors; heparin; Intercellular Signaling Peptides and Proteins - blood; leukocytes; Male; Plasma - chemistry; protein content; Reference Values; Reproducibility of Results; Serum - chemistry; Specific Pathogen-Free Organisms

OBJECTIVE To evaluate a feline-specific multiplex, bead-based assay system for detection of recombinant and native proteins in serum samples and in EDTA-treated and heparinized plasma samples. SAMPLE Serum samples and EDTA-treated and heparinized plasma samples from 30 sick cats and 9 healthy client...