Modulation of biophysical properties of nucleocapsid protein in the mutant spectrum of SARS-CoV-2

• Published in: eLife Vol. 13
• Main Authors: Nguyen, Ai, Zhao, Huaying, Myagmarsuren, Dulguun, Srinivasan, Sanjana, Wu, Di, Chen, Jiji, Piszczek, Grzegorz, Schuck, Peter
• Format: Journal Article
• Language: English
• Published: England eLife Sciences Publications Ltd 28.06.2024
• Subjects: Amino acids; biophysical fitness landscape; Coronavirus Nucleocapsid Proteins - chemistry; Coronavirus Nucleocapsid Proteins - genetics; Coronavirus Nucleocapsid Proteins - metabolism; Coronaviruses; COVID-19 - genetics; COVID-19 - virology; Evolution; Functional plasticity; Genetic diversity; genotype-phenotype relationship; Humans; intrinsically disordered protein; Intrinsically Disordered Proteins - chemistry; Intrinsically Disordered Proteins - genetics; Intrinsically Disordered Proteins - metabolism; mutant spectrum; Mutation; Nucleocapsid Proteins - chemistry; Nucleocapsid Proteins - genetics; Nucleocapsid Proteins - metabolism; Nucleocapsids; Phenotypes; Phenotypic plasticity; Phenotypic variations; Phosphoproteins - chemistry; Phosphoproteins - genetics; Phosphoproteins - metabolism; Physicochemical properties; Point mutation; protein evolution; Protein interaction; Protein Stability; Protein structure; Proteins; RNA viruses; SARS-CoV-2; SARS-CoV-2 - chemistry; SARS-CoV-2 - genetics; SARS-CoV-2 - metabolism; Secondary structure; Severe acute respiratory syndrome coronavirus 2; Thermodynamics; mutant spectrum; SARS-CoV-2; viruses; intrinsically disordered protein; protein evolution; infectious disease; biophysical fitness landscape; microbiology; genotype-phenotype relationship; structural biology; molecular biophysics

Genetic diversity is a hallmark of RNA viruses and the basis for their evolutionary success. Taking advantage of the uniquely large genomic database of SARS-CoV-2, we examine the impact of mutations across the spectrum of viable amino acid sequences on the biophysical phenotypes of the highly expres...