A forward genetic screen identifies a negative regulator of rapid Ca2+-dependent cell egress (MS1) in the intracellular parasite Toxoplasma gondii

• Published in: The Journal of biological chemistry Vol. 292; no. 18; pp. 7662 - 7674
• Main Authors: McCoy, James M., Stewart, Rebecca J., Uboldi, Alessandro D., Li, Dongdi, Schröder, Jan, Scott, Nicollas E., Papenfuss, Anthony T., Lehane, Adele M., Foster, Leonard J., Tonkin, Christopher J.
• Format: Journal Article
• Language: English
• Published: 11200 Rockville Pike, Suite 302, Rockville, MD 20852-3110, U.S.A Elsevier Inc 05.05.2017; American Society for Biochemistry and Molecular Biology
• Subjects: apicomplexa; Ca2+ signalling; forward genetic screen; host cell egress; Microbiology; molecular genetics; parasite; proteomics; signaling; Toxoplasma gondii; molecular genetics; parasite; signaling; Toxoplasma gondii; proteomics; apicomplexa; forward genetic screen; Ca2+ signalling; host cell egress

Toxoplasma gondii, like all apicomplexan parasites, uses Ca2+ signaling pathways to activate gliding motility to power tissue dissemination and host cell invasion and egress. A group of “plant-like” Ca2+-dependent protein kinases (CDPKs) transduces cytosolic Ca2+ flux into enzymatic activity, but ho...