A forward genetic screen identifies a negative regulator of rapid Ca2+-dependent cell egress (MS1) in the intracellular parasite Toxoplasma gondii

Toxoplasma gondii, like all apicomplexan parasites, uses Ca2+ signaling pathways to activate gliding motility to power tissue dissemination and host cell invasion and egress. A group of “plant-like” Ca2+-dependent protein kinases (CDPKs) transduces cytosolic Ca2+ flux into enzymatic activity, but ho...

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Published inThe Journal of biological chemistry Vol. 292; no. 18; pp. 7662 - 7674
Main Authors McCoy, James M., Stewart, Rebecca J., Uboldi, Alessandro D., Li, Dongdi, Schröder, Jan, Scott, Nicollas E., Papenfuss, Anthony T., Lehane, Adele M., Foster, Leonard J., Tonkin, Christopher J.
Format Journal Article
LanguageEnglish
Published 11200 Rockville Pike, Suite 302, Rockville, MD 20852-3110, U.S.A Elsevier Inc 05.05.2017
American Society for Biochemistry and Molecular Biology
Subjects
apicomplexa
Ca2+ signalling
forward genetic screen
host cell egress
Microbiology
molecular genetics
parasite
proteomics
signaling
Toxoplasma gondii
molecular genetics
parasite
signaling
Toxoplasma gondii
proteomics
apicomplexa
forward genetic screen
Ca2+ signalling
host cell egress
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Summary:Toxoplasma gondii, like all apicomplexan parasites, uses Ca2+ signaling pathways to activate gliding motility to power tissue dissemination and host cell invasion and egress. A group of “plant-like” Ca2+-dependent protein kinases (CDPKs) transduces cytosolic Ca2+ flux into enzymatic activity, but how they function is poorly understood. To investigate how Ca2+ signaling activates egress through CDPKs, we performed a forward genetic screen to isolate gain-of-function mutants from an egress-deficient cdpk3 knockout strain. We recovered mutants that regained the ability to egress from host cells that harbored mutations in the gene Suppressor of Ca2+-dependent Egress 1 (SCE1). Global phosphoproteomic analysis showed that SCE1 deletion restored many Δcdpk3-dependent phosphorylation events to near wild-type levels. We also show that CDPK3-dependent SCE1 phosphorylation is required to relieve its suppressive activity to potentiate egress. In summary, our work has uncovered a novel component and suppressor of Ca2+-dependent cell egress during Toxoplasma lytic growth.
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Edited by Joseph Jez
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M117.775114