DNA Methylation in CYP82E4 Regulates Nicotine Conversion of Nicotiana tabacum

• Published in: Plant, cell and environment Vol. 48; no. 7; pp. 5330 - 5341
• Main Authors: Wang, Yaqi, Zhang, Xingzi, Zhang, Fang, Cheng, Lirui, Jiang, Caihong, Yang, Aiguo, Li, Fengxia
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.07.2025
• Subjects: alkaloid; Azacitidine - pharmacology; Bisulfite; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; Deoxyribonucleic acid; DNA; DNA methylase; DNA methylation; DNA Methylation - genetics; DNA methylation inhibitors; DNA methyltransferase; epigenetic modification; Gene Expression Regulation, Plant; Genotype & phenotype; Methylase; Mutation; Nicotiana - enzymology; Nicotiana - genetics; Nicotiana - metabolism; Nicotine; Nicotine - analogs & derivatives; Nicotine - metabolism; nicotine conversion; nicotine N‐demethylase; Nornicotine; Phenotype; Phenotypes; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Tobacco; nicotine conversion; DNA methylation inhibitors; DNA methylation; DNA methyltransferase; epigenetic modification; alkaloid; nicotine N‐demethylase

ABSTRACT Nornicotine content is very low in tobacco, accounting for less than 5% of total alkaloids. Nicotine conversion refers to the process by which nornicotine is synthesised spontaneously and in large quantities from nicotine. CYP82E4 is the only key enzyme gene involved in nicotine conversion,...