Relationship between acrylamide concentration and enzymatic activity in an improved single fibrin zymogram gel system

Based on the zymography analysis, Bacillus sp. DJ-4 (screened from Doen-Jang, a Korean traditional fermented food) secretes seven extracellular fibrinolytic enzymes (EFEs; 68, 64, 55, 45, 33, 27, and 13 kDa) in culture broth. These seven EFEs were analyzed by newly applied SDSfibrin zymography combi...

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Published inBMB reports Vol. 35; no. 2; pp. 236 - 238
Main Authors Choi, Nack-Shick, Kim, Byoung-Young, Lee, Jin-Young, Yoon, Kab-Seog, Han, Kyoung-Yoen, Kim, Seung-Ho
Format Journal Article
LanguageEnglish
Published Korea (South) 31.03.2002
Subjects
Acrylamide
Animals
Bacillus - enzymology
Cattle
Electrophoresis, Polyacrylamide Gel - methods
Endopeptidases - chemistry
Endopeptidases - isolation & purification
Endopeptidases - metabolism
Fibrin
Fibrinolysis
Food Microbiology
In Vitro Techniques
Sodium Dodecyl Sulfate
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Abstract Based on the zymography analysis, Bacillus sp. DJ-4 (screened from Doen-Jang, a Korean traditional fermented food) secretes seven extracellular fibrinolytic enzymes (EFEs; 68, 64, 55, 45, 33, 27, and 13 kDa) in culture broth. These seven EFEs were analyzed by newly applied SDSfibrin zymography combined with gradient polyacrylamide (SDS-FZGP). This improved gel system was used with a 5-20% acrylamide gradient in a fibrin zymogram gel for the separation of proteins with molecular masses from below 10 kDa to over 100 kDa on one gel plate. Using this system, high molecular weight bands (HMWBs) were clearly and sharply resolved. We also examined the relationship between an acrylamide concentration and the enzymatic activity of EFE using densitometric analysis.
AbstractList Based on the zymography analysis, Bacillus sp. DJ-4 (screened from Doen-Jang, a Korean traditional fermented food) secretes seven extracellular fibrinolytic enzymes (EFEs; 68, 64, 55, 45, 33, 27, and 13 kDa) in culture broth. These seven EFEs were analyzed by newly applied SDSfibrin zymography combined with gradient polyacrylamide (SDS-FZGP). This improved gel system was used with a 5-20% acrylamide gradient in a fibrin zymogram gel for the separation of proteins with molecular masses from below 10 kDa to over 100 kDa on one gel plate. Using this system, high molecular weight bands (HMWBs) were clearly and sharply resolved. We also examined the relationship between an acrylamide concentration and the enzymatic activity of EFE using densitometric analysis.
Author Yoon, Kab-Seog
Kim, Seung-Ho
Kim, Byoung-Young
Lee, Jin-Young
Choi, Nack-Shick
Han, Kyoung-Yoen
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Cites_doi 10.1016/0003-2697(76)90527-3
10.1006/abio.1999.4080
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SubjectTerms Acrylamide
Animals
Bacillus - enzymology
Cattle
Electrophoresis, Polyacrylamide Gel - methods
Endopeptidases - chemistry
Endopeptidases - isolation & purification
Endopeptidases - metabolism
Fibrin
Fibrinolysis
Food Microbiology
In Vitro Techniques
Sodium Dodecyl Sulfate
Title Relationship between acrylamide concentration and enzymatic activity in an improved single fibrin zymogram gel system
URI https://www.ncbi.nlm.nih.gov/pubmed/12297035
Volume 35
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