A novel multi-affinity tag system to produce high levels of soluble and biotinylated proteins in Escherichia coli - Ashraf, S.Salman; Benson, R.Edward; Payne, E.Sturgis; Halbleib, Cale M; Grøn, Hanne (Protein expression and purification, 2004)
Keywords: AviTag; Base Sequence; Biotinylation; Carrier Proteins - genetics; Carrier Proteins - metabolism; Culture Media; DNA Gyrase - chemistry; DNA Gyrase - genetics; DNA Gyrase - metabolism; E. coli; Ef-Tu; Escherichia coli - genetics; Escherichia coli Proteins - biosynthesis; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - isolation & purification; Fusion proteins; Genetic Vectors; Glucocorticoid receptor; GyrA; His-tag; Histidine - chemistry; Humans; In vivo biotinylation; Maltose binding protein; Maltose-Binding Proteins; Models, Biological; Molecular Sequence Data; Peptide Elongation Factor Tu - chemistry; Peptide Elongation Factor Tu - genetics; Peptide Elongation Factor Tu - metabolism; Plasmids; Protein solubility; Receptors, Cytoplasmic and Nuclear - genetics; Receptors, Cytoplasmic and Nuclear - metabolism; Receptors, Glucocorticoid - genetics; Receptors, Glucocorticoid - metabolism; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - isolation & purification; Recombinant protein expression; Solubility; TufB; In vivo biotinylation; GyrA; Ef-Tu; His-tag; Glucocorticoid receptor; TufB; Protein solubility; AviTag; Fusion proteins; E. coli; Maltose binding protein; Recombinant protein expression