Microenvironment improvement protocol for the sensitive spectrofluorimetric determination of an hepatitis C virus antiviral (Simeprevir): application to human plasma
Simeprevir (SPV) is a powerful antihepatitis C virus agent that was newly introduced into the pharmaceutical market. We here established and validated an easy, simple, and sensitive spectrofluorimetric method for its estimation at λem 427 nm (λex 337 nm). The suggested procedure was based on two tim...
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| Published in | Luminescence (Chichester, England) Vol. 35; no. 3; pp. 393 - 399 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
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01.05.2020
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| Abstract | Simeprevir (SPV) is a powerful antihepatitis C virus agent that was newly introduced into the pharmaceutical market. We here established and validated an easy, simple, and sensitive spectrofluorimetric method for its estimation at λem 427 nm (λex 337 nm). The suggested procedure was based on two times enhancement in the original emission of SPV through modifying its microenvironment in buffered aqueous solution by adding Triton X‐100. The relationship between the concentration of SPV and the observed fluorescence intensity was linear in the range 0.06–1.0 μg ml−1 with a correlation coefficient of 0.9997. The limits of detection and quantitation were 21 and 64 ng ml−1, respectively. The present method was effectively applied to quantify SPV content in pharmaceutical tablets and human plasma spiked with the drug with no interference from tablet excipients or plasma components. |
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| AbstractList | Simeprevir (SPV) is a powerful antihepatitis C virus agent that was newly introduced into the pharmaceutical market. We here established and validated an easy, simple, and sensitive spectrofluorimetric method for its estimation at λem 427 nm (λex 337 nm). The suggested procedure was based on two times enhancement in the original emission of SPV through modifying its microenvironment in buffered aqueous solution by adding Triton X‐100. The relationship between the concentration of SPV and the observed fluorescence intensity was linear in the range 0.06–1.0 μg ml−1 with a correlation coefficient of 0.9997. The limits of detection and quantitation were 21 and 64 ng ml−1, respectively. The present method was effectively applied to quantify SPV content in pharmaceutical tablets and human plasma spiked with the drug with no interference from tablet excipients or plasma components. Abstract Simeprevir (SPV) is a powerful antihepatitis C virus agent that was newly introduced into the pharmaceutical market. We here established and validated an easy, simple, and sensitive spectrofluorimetric method for its estimation at λ em 427 nm (λ ex 337 nm). The suggested procedure was based on two times enhancement in the original emission of SPV through modifying its microenvironment in buffered aqueous solution by adding Triton X‐100. The relationship between the concentration of SPV and the observed fluorescence intensity was linear in the range 0.06–1.0 μg ml −1 with a correlation coefficient of 0.9997. The limits of detection and quantitation were 21 and 64 ng ml −1 , respectively. The present method was effectively applied to quantify SPV content in pharmaceutical tablets and human plasma spiked with the drug with no interference from tablet excipients or plasma components. Simeprevir (SPV) is a powerful antihepatitis C virus agent that was newly introduced into the pharmaceutical market. We here established and validated an easy, simple, and sensitive spectrofluorimetric method for its estimation at λ 427 nm (λ 337 nm). The suggested procedure was based on two times enhancement in the original emission of SPV through modifying its microenvironment in buffered aqueous solution by adding Triton X-100. The relationship between the concentration of SPV and the observed fluorescence intensity was linear in the range 0.06-1.0 μg ml with a correlation coefficient of 0.9997. The limits of detection and quantitation were 21 and 64 ng ml , respectively. The present method was effectively applied to quantify SPV content in pharmaceutical tablets and human plasma spiked with the drug with no interference from tablet excipients or plasma components. |
| Author | Derayea, Sayed M. A. El Hamd, Mohamed Ali, Sayed Samir, Ebtihal |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31849179$$D View this record in MEDLINE/PubMed |
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| DOI | 10.1002/bio.3739 |
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| References | 2017; 7 2015; 15 2014; 958 2015; 5 1986; 113 1974; 96 2011; 50 2019 1974 2016; 121 1983 2005 2016; 125 2019; 206 2018; 33 2003; 785 2018; 190 2016; 26 2019; 210 1996; 6 e_1_2_6_10_1 Martin A. N. (e_1_2_6_20_1) 1983 e_1_2_6_9_1 e_1_2_6_8_1 e_1_2_6_19_1 e_1_2_6_5_1 e_1_2_6_4_1 e_1_2_6_7_1 e_1_2_6_6_1 e_1_2_6_13_1 e_1_2_6_14_1 e_1_2_6_3_1 e_1_2_6_11_1 e_1_2_6_2_1 e_1_2_6_12_1 e_1_2_6_22_1 e_1_2_6_17_1 e_1_2_6_18_1 Guideline I. H. T. (e_1_2_6_21_1) 2005 e_1_2_6_15_1 e_1_2_6_16_1 |
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| Snippet | Simeprevir (SPV) is a powerful antihepatitis C virus agent that was newly introduced into the pharmaceutical market. We here established and validated an easy,... Abstract Simeprevir (SPV) is a powerful antihepatitis C virus agent that was newly introduced into the pharmaceutical market. We here established and validated... |
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| SubjectTerms | Antiviral agents Aqueous solutions Blood plasma Correlation coefficient Correlation coefficients Fluorescence Hepatitis Hepatitis C human plasma pharmaceutical analysis Pharmaceuticals Quantitation Simeprevir spectrofluorimetry surfactants Viruses |
| Title | Microenvironment improvement protocol for the sensitive spectrofluorimetric determination of an hepatitis C virus antiviral (Simeprevir): application to human plasma |
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