Formation of oxidative and non‐oxidative dimers in metallothioneins: Implications for charge‐state analysis for structural determination

• Published in: Rapid communications in mass spectrometry Vol. 31; no. 24; pp. 2118 - 2124
• Main Authors: Irvine, Gordon W., Heinlein, Lina, Renaud, Justin B., Sumarah, Mark W., Stillman, Martin J.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 30.12.2017
• Subjects: Cysteine - analysis; Cysteine - chemistry; Cysteine - metabolism; Dimerization; Dimers; High resolution; Ionization; Ions; Mass spectrometers; Mass spectrometry; Metallothionein - analysis; Metallothionein - chemistry; Metallothionein - metabolism; Oxidation; Oxidation-Reduction; Protein Conformation; Proteins; Spectrometers; Spectrometry, Mass, Electrospray Ionization; Structural analysis; Zinc

Rationale Metallothioneins (MTs) are a class of dynamic proteins that have been investigated extensively using mass spectrometric methods due to their amenability to ionization. Here we detect the formation of oxidative and non‐oxidative MT dimers using high‐resolution mass spectrometry (HRMS) which...