Molecular cloning, gene expression analysis, and in silico characterization of UDP-N-acetylglucosamine pyrophosphorylase from Bombyx mori

• Published in: Biotechnology and applied biochemistry Vol. 66; no. 5; p. 880
• Main Authors: Palaka, Bhagath Kumar, Velmurugan Ilavarasi, Anbumani, Sapam, Tuleshwori Devi, Kotapati, Kasi Viswanath, Nallala, Venkata Satyanarayana, Khan, Mohd Babu, Ampasala, Dinakara Rao
• Format: Journal Article
• Language: English
• Published: United States 01.09.2019
• Subjects: Animals; Bombyx - enzymology; Bombyx - genetics; Cloning, Molecular; Computer Simulation; Gene Expression Regulation, Enzymologic - genetics; Humans; Molecular Docking Simulation; Nucleotidyltransferases - chemistry; Nucleotidyltransferases - genetics; Nucleotidyltransferases - metabolism; Protein Conformation; UDP-N-acetylglucosamine pyrophosphorylase; Docking and MD simulations; insect molting; PCR; Hormonal regulation

The present study was aimed to explore the molecular and structural features of UDP-N-acetylglucosamine pyrophosphorylase of Bombyx mori (BmUAP), an essential enzyme for chitin synthesis in insects. The BmUAP cDNA sequence was cloned and expression profiles were monitored during the molting and feed...