Molecular cloning, gene expression analysis, and in silico characterization of UDP-N-acetylglucosamine pyrophosphorylase from Bombyx mori

• Published in: Biotechnology and applied biochemistry Vol. 66; no. 5; p. 880
• Main Authors: Palaka, Bhagath Kumar, Velmurugan Ilavarasi, Anbumani, Sapam, Tuleshwori Devi, Kotapati, Kasi Viswanath, Nallala, Venkata Satyanarayana, Khan, Mohd Babu, Ampasala, Dinakara Rao
• Format: Journal Article
• Language: English
• Published: United States 01.09.2019
• Subjects: Animals; Bombyx - enzymology; Bombyx - genetics; Cloning, Molecular; Computer Simulation; Gene Expression Regulation, Enzymologic - genetics; Humans; Molecular Docking Simulation; Nucleotidyltransferases - chemistry; Nucleotidyltransferases - genetics; Nucleotidyltransferases - metabolism; Protein Conformation; UDP-N-acetylglucosamine pyrophosphorylase; Docking and MD simulations; insect molting; PCR; Hormonal regulation
• DOI: 10.1002/bab.1802

The present study was aimed to explore the molecular and structural features of UDP-N-acetylglucosamine pyrophosphorylase of Bombyx mori (BmUAP), an essential enzyme for chitin synthesis in insects. The BmUAP cDNA sequence was cloned and expression profiles were monitored during the molting and feeding stages of silkworm larvae. The effect of 20-hydroxyecdysone (20E) on BmUAP expression, and on silkworm molting was studied, which revealed that 20E regulates its expression. Multiple sequence alignment of various pyrophosphorylases revealed that the residues N223, G290, N327, and K407 of human UAP (PDB ID: 1JV1) were found to be highly conserved in BmUAP and all other eukaryotic UAPs considered for the study. Phylogenetic analysis inferred that the UAPs possess discrete variations in primary structure among different insect Orders while sharing good identity between species of the Order. The structure of BmUAP was predicted and its interactions with uridine triphosphate, N-acetylglucosamine-1-phosphate, and UDP-N-acetylglucosamine were analyzed. Virtual screening with a library of natural compounds resulted in five potential hits with good binding affinities. On further analysis, these five hits were found to be mimicking substrate and product, in inducing conformational changes in the active site. This work provides crucial information on molecular interactions and structural dynamics of insect UAPs.