Long and Accurate: How HiFi Sequencing is Transforming Genomics

• Published in: Genomics, proteomics & bioinformatics Vol. 23; no. 1
• Main Authors: Wang (王博), Bo, Jia (贾鹏), Peng, Gao (高胜寒), Shenghan, Zhao (赵焕焕), Huanhuan, Zheng (郑高洋), Gaoyang, Xu (许林峰), Linfeng, Ye (叶凯), Kai
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 10.05.2025
• Subjects: Animals; Epigenomics; Genomics - methods; High-Throughput Nucleotide Sequencing - methods; Humans; Review; Sequence Analysis, DNA - methods; Long-read sequencing; Genome assembly; Centromere; Complex genomic region; Variant detection

Recent developments in PacBio high-fidelity (HiFi) sequencing technologies have transformed genomic research, with circular consensus sequencing now achieving 99.9% accuracy for long (up to 25 kb) single-molecule reads. This method circumvents biases intrinsic to amplification-based approaches, enab...