Understanding the impact of different post-digestion enzymatic inhibition methods and storage conditions on standardized gastrointestinal model (INFOGEST): A case study of Pleurotus ostreatus mushroom biomass

• Published in: Food research international Vol. 211; p. 116432
• Main Authors: Araújo-Rodrigues, Helena, Salsinha, Ana Sofia, Coscueta, Ezequiel R., Relvas, João Bettencourt, Tavaria, Freni K., Pintado, Manuela E.
• Format: Journal Article
• Language: English
• Published: Canada Elsevier Ltd 01.06.2025
• Subjects: Antioxidant activity; Biomass; Carbohydrates; case studies; Digestion; digestive tract; Enzymatic inactivation; enzyme activity; enzyme inactivation; enzyme inhibition; Enzyme Inhibitors - pharmacology; Fatty acids; food matrix; food research; Food Storage - methods; Freeze Drying; Freezing; gastrointestinal system; Gastrointestinal Tract - metabolism; glucans; Glucans - metabolism; heat inactivation; Hydrogen-Ion Concentration; INFOGEST protocol; lipids; Macronutrient groups assessment; Mushroom biomass; mushrooms; peptides; Pleurotus - chemistry; Pleurotus - enzymology; Pleurotus ostreatus; Post-digestion storage; Proteins and peptides; solubility; Total phenolic compounds; Carbohydrates; Total phenolic compounds; Proteins and peptides; Antioxidant activity; Macronutrient groups assessment; INFOGEST protocol; Enzymatic inactivation; Mushroom biomass; Fatty acids; Post-digestion storage

INFOGEST became the standard digestion protocol to address inconsistencies in gastrointestinal tract (GIT) digestion protocols. However, sample collection, enzymatic inactivation methods, and storage conditions can significantly impact the accurate assessment of food compounds. This study investigat...