Reprogrammed Peripheral Blood Mononuclear Cells are Able to Survive Longer in Irradiated Female Mice

• Published in: Molecular biotechnology Vol. 55; no. 2; pp. 111 - 119
• Main Authors: Ruan, Guang-Ping, Han, Yi-Bing, Ruan, Guang-Hong, Zhu, Xiang-Qing, Yao, Xiang, Pang, Rong-Qing, Cai, Xue-Ming, Wang, Jin-Xiang, He, Jie, Zhao, Jing, Zhu, Guang-Xu, Xu, Xin-Ming, Pan, Xing-Hua
• Format: Journal Article
• Language: English
• Published: Boston Springer-Verlag 01.10.2013; Springer US; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biological Techniques; Biotechnology; Cell Biology; Cell Survival; Cells, Cultured; Cellular Reprogramming - physiology; Chemistry; Chemistry and Materials Science; Epigenetics; Female; fibroblasts; fish; Fishes; fluorescence in situ hybridization; Genetic research; Human Genetics; In Situ Hybridization, Fluorescence; Induced Pluripotent Stem Cells - physiology; Induced Pluripotent Stem Cells - transplantation; Irradiation; Leukocytes, Mononuclear - physiology; Leukocytes, Mononuclear - radiation effects; Male; Mice; Mice, Inbred C57BL; Mice, Transgenic; mononuclear leukocytes; Oocytes; Protein Science; Rodents; somatic cells; spleen; Spleen - metabolism; Stem cells; Whole-Body Irradiation; Y Chromosome; Natural active substances; Transplantation; Survive; Induced multipotent stem cells; Fish oocytes extracts
• ISSN: 1073-6085; 1559-0305
• DOI: 10.1007/s12033-013-9661-9

Induced multipotent stem (iMS) cells are originated from somatic cells and become multipotent by genetic and/or epigenetic modifications. Previous studies have shown that the fish oocytes extracts (FOE) can induce skin fibroblast cells into iMS cells. In this study, we aim to determine whether FOE can similarly induce mouse peripheral blood mononuclear cells (PBMCs) into the iMS state and if so, whether they can survive longer when they are transplanted into the irradiation female mice. PBMCs of GFP-transgenic male mice were cultured and transiently reprogrammed by FOE. They were deemed reaching the iMS state after detection of expression of stem cell markers. The iMS-like PBMCs were transplanted into female C57BL mice by tail vein injection. The spleen wet weights as well as numbers of colonies of the recipient mice were examined. The results showed the spleen wet weights and numbers of spleen colonies of FOE-induced group were all significantly higher than those of the non-induced group and negative control group. On day 90 after transplantation, FISH analysis detected the presence of Y chromosome in the induced group, but not of the other groups. The current findings demonstrate that FOE-induced PBMCs are able to survive longer in irradiated female mice.