Vitreous tissue cryopreservation using a blood vessel model and cryomacroscopy for scale-up studies: Observations and mathematical modeling

• Published in: Cryobiology Vol. 117; p. 104976
• Main Authors: Taylor, Michael J., Solanki, Prem K., Chen, Zhenzhen, Baicu, Simona, Crossley, Christina, Greene, Elizabeth D., Campbell, Lia H., Brockbank, Kelvin G.M., Rabin, Yoed
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.12.2024
• Subjects: Animals; Blood vessels; Blood Vessels - physiology; Contractility; cooling; cryobiology; Cryomacroscopy; cryopreservation; Cryopreservation - methods; cryoprotectants; Cryoprotective Agents - chemistry; Cryoprotective Agents - pharmacology; crystallization; glass; Ice; Ice-free cryopreservation; mechanical stress; Models, Theoretical; Physical effects; Rabbits; sucrose; Sucrose - chemistry; survival rate; Swine; Thermo-mechanics; toxicity; Viability; Vitrification; Cryomacroscopy; Thermo-mechanics; Physical effects; Vitrification; Contractility; Blood vessels; Ice-free cryopreservation; Viability

Successful long-term cryobanking of multicellular tissues and organs at deep subzero temperatures calls for the avoidance of ice cryoinjury by reliance upon ice-free cryopreservation techniques. However, the quality of the cryopreserved material is the direct result of its ability to survive a host...