Isolation and characterization of nitrogen-fixing bacteria of the genus Azospirillum from the soil of a Sphagnum peat bog
he presence of nitrogen-fixing bacteria of the genus Azospirillum in the soils of acidic raised Sphagnum bogs is revealed for the first time. Three Azospirillum strains, B2, B21, and B22, were isolated as a component of methane-oxidizing enrichment cultures, whereas attempts to isolate them directly...
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Published in | Microbiology (New York) Vol. 76; no. 1; pp. 93 - 101 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
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Nauka/Interperiodica
01.01.2007
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Abstract | he presence of nitrogen-fixing bacteria of the genus Azospirillum in the soils of acidic raised Sphagnum bogs is revealed for the first time. Three Azospirillum strains, B2, B21, and B22, were isolated as a component of methane-oxidizing enrichment cultures, whereas attempts to isolate them directly from peat samples have failed. The results of comparative analysis of the nucleotide sequences of 16S rRNA genes, DNA-DNA hybridization, and the analysis of the sequences of the functional genes encoding nitrogenase and ribulose-1, 5-bisphosphate carboxylase reveal that all the newly obtained strains can be classified as Azospirillum lipoferum. Yet, unlike A. lipoferum, the isolates do not require biotin and utilize sucrose, inositol, and glycerol for growth. The cell morphology of strain B2 differs from that of the type strain and strains B21 and B22. The results obtained indicate the variability of morphological, physiological, and biochemical properties in closely related Azospirillum strains and suggest the existence of metabolic relationships between methanotrophic bacteria and the representatives of the genus Azospirillum under peat bog conditions. |
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AbstractList | he presence of nitrogen-fixing bacteria of the genus Azospirillum in the soils of acidic raised Sphagnum bogs is revealed for the first time. Three Azospirillum strains, B2, B21, and B22, were isolated as a component of methane-oxidizing enrichment cultures, whereas attempts to isolate them directly from peat samples have failed. The results of comparative analysis of the nucleotide sequences of 16S rRNA genes, DNA-DNA hybridization, and the analysis of the sequences of the functional genes encoding nitrogenase and ribulose-1, 5-bisphosphate carboxylase reveal that all the newly obtained strains can be classified as Azospirillum lipoferum. Yet, unlike A. lipoferum, the isolates do not require biotin and utilize sucrose, inositol, and glycerol for growth. The cell morphology of strain B2 differs from that of the type strain and strains B21 and B22. The results obtained indicate the variability of morphological, physiological, and biochemical properties in closely related Azospirillum strains and suggest the existence of metabolic relationships between methanotrophic bacteria and the representatives of the genus Azospirillum under peat bog conditions. he presence of nitrogen-fixing bacteria of the genus Azospirillum in the soils of acidic raised Sph agnum bogs is revealed for the first time. Three Azospirillum strains, B2, B21, and B22, were isolated as a com ponent of methane-oxidizing enrichment cultures, whereas attempts to isolate them directly from peat samples have failed. The results of comparative analysis of the nucleotide sequences of 16S rRNA genes, DNA-DNA hybridization, and the analysis of the sequences of the functional genes encoding nitrogenase and ribulose-1, 5-bisphosphate carboxylase reveal that all the newly obtained strains can be classified as Azospirillum lipof-erum. Yet, unlike A. lipoferum, the isolates do not require biotin and utilize sucrose, inositol, and glycerol for growth. The cell morphology of strain B2 differs from that of the type strain and strains B21 and B22. The results obtained indicate the variability of morphological, physiological, and biochemical properties in closely related Azospirillum strains and suggest the existence of metabolic relationships between methanotrophic bac teria and the representatives of the genus Azospirillum under peat bog conditions. |
Author | Spiridonova, E. M Tourova, T. P Kravchenko, I. K Doroshenko, E. V Boulygina, E. S |
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Cites_doi | 10.1099/mic.0.26585-0 10.1111/j.1574-6976.2000.tb00552.x 10.1139/m78-160 10.1094/MPMI.2001.14.4.555 10.1139/w04-007 10.1007/BF01577194 10.1139/m79-100 10.1007/s11104-004-1522-7 10.1128/AEM.41.1.203-206.1981 10.1139/w04-035 10.1128/AEM.64.11.4384-4389.1998 |
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References | O. Steenhoudt (1013_CR6) 2000; 24 M.M. Fisher (1013_CR21) 1998; 64 S. Burdman (1013_CR7) 2001; 14 J. Dobereiner (1013_CR19) 1991 M.F. Cohen (1013_CR24) 2004; 50 L.I. Pozdnyakova (1013_CR12) 1988; 57 E.A. Caceres (1013_CR15) 1983; 4 S.E. Belova (1013_CR3) 2006; 75 S.N. Dedysh (1013_CR4) 2004; 150 1013_CR20 E.M. Spiridonova (1013_CR18) 2004; 73 N. Tejera (1013_CR9) 2005; 270 J.J. Tarrand (1013_CR10) 1978; 24 D.J. Lane (1013_CR17) 1991 M.E. Tyler (1013_CR5) 1979; 25 K. Haantela (1013_CR11) 1981; 141 A.I. Marusina (1013_CR16) 2001; 70 Yu.P. Fedonenko (1013_CR8) 2001; 70 I.K. Kravchenko (1013_CR1) 2003; 72 A.V. Golovchenko (1013_CR2) 2002; 35 Y. Bashan (1013_CR22) 2004; 50 J. Dobereiner (1013_CR13) 1980 N.A. Slobodova (1013_CR14) 2006; 75 K.V.B.R. Tilak (1013_CR23) 1986; 13 |
References_xml | – volume: 150 start-page: 1301 year: 2004 ident: 1013_CR4 publication-title: Microbiology (UK) doi: 10.1099/mic.0.26585-0 contributor: fullname: S.N. Dedysh – volume: 70 start-page: 86 issue: 1 year: 2001 ident: 1013_CR16 publication-title: Mikrobiologiya contributor: fullname: A.I. Marusina – start-page: 115 volume-title: 16S/23S RRNA Sequencing, Nucleic Acid Techniques in Bacterial Systematics year: 1991 ident: 1013_CR17 contributor: fullname: D.J. Lane – volume: 73 start-page: 377 issue: 3 year: 2004 ident: 1013_CR18 publication-title: Mikrobiologiya contributor: fullname: E.M. Spiridonova – volume: 24 start-page: 487 year: 2000 ident: 1013_CR6 publication-title: FEMS Microbiol. Rev. doi: 10.1111/j.1574-6976.2000.tb00552.x contributor: fullname: O. Steenhoudt – volume: 57 start-page: 275 issue: 2 year: 1988 ident: 1013_CR12 publication-title: Mikrobiologiya contributor: fullname: L.I. Pozdnyakova – volume: 70 start-page: 384 issue: 3 year: 2001 ident: 1013_CR8 publication-title: Mikrobiologiya contributor: fullname: Yu.P. Fedonenko – volume: 24 start-page: 967 year: 1978 ident: 1013_CR10 publication-title: Can. J. Microbiol. doi: 10.1139/m78-160 contributor: fullname: J.J. Tarrand – volume: 35 start-page: 1468 issue: 12 year: 2002 ident: 1013_CR2 publication-title: Pochvovedenie contributor: fullname: A.V. Golovchenko – ident: 1013_CR20 – volume: 14 start-page: 555 year: 2001 ident: 1013_CR7 publication-title: Mol. Plant. Microbe. Interact doi: 10.1094/MPMI.2001.14.4.555 contributor: fullname: S. Burdman – volume: 50 start-page: 291 year: 2004 ident: 1013_CR24 publication-title: Can. J. Microbiol. doi: 10.1139/w04-007 contributor: fullname: M.F. Cohen – volume: 13 start-page: 291 year: 1986 ident: 1013_CR23 publication-title: Curr. Microbiol. doi: 10.1007/BF01577194 contributor: fullname: K.V.B.R. Tilak – volume: 25 start-page: 4673 year: 1979 ident: 1013_CR5 publication-title: Can. J. Microbiol. doi: 10.1139/m79-100 contributor: fullname: M.E. Tyler – volume: 75 start-page: 110 issue: 1 year: 2006 ident: 1013_CR3 publication-title: Mikrobiologiya contributor: fullname: S.E. Belova – volume: 72 start-page: 111 issue: 1 year: 2003 ident: 1013_CR1 publication-title: Mikrobiologiya contributor: fullname: I.K. Kravchenko – volume: 270 start-page: 223 year: 2005 ident: 1013_CR9 publication-title: Plant Soil doi: 10.1007/s11104-004-1522-7 contributor: fullname: N. Tejera – volume: 141 start-page: 203 year: 1981 ident: 1013_CR11 publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.41.1.203-206.1981 contributor: fullname: K. Haantela – volume: 4 start-page: 990 year: 1983 ident: 1013_CR15 publication-title: Appl. Environ. Microbiol. contributor: fullname: E.A. Caceres – volume: 50 start-page: 521 year: 2004 ident: 1013_CR22 publication-title: Can. J. Microbiol. doi: 10.1139/w04-035 contributor: fullname: Y. Bashan – start-page: 535 volume-title: Methods of Evaluation of Biological Nitrogen Fixation year: 1980 ident: 1013_CR13 contributor: fullname: J. Dobereiner – volume: 75 start-page: 397 issue: 3 year: 2006 ident: 1013_CR14 publication-title: Mikrobiologiya contributor: fullname: N.A. Slobodova – start-page: 2236 volume-title: The Genera Azospirillum and Herbaspirillum, The Prokaryotes year: 1991 ident: 1013_CR19 contributor: fullname: J. Dobereiner – volume: 64 start-page: 4384 year: 1998 ident: 1013_CR21 publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.64.11.4384-4389.1998 contributor: fullname: M.M. Fisher |
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Snippet | he presence of nitrogen-fixing bacteria of the genus Azospirillum in the soils of acidic raised Sphagnum bogs is revealed for the first time. Three... he presence of nitrogen-fixing bacteria of the genus Azospirillum in the soils of acidic raised Sph agnum bogs is revealed for the first time. Three... |
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SubjectTerms | acid soils Azospirillum Azospirillum lipoferum biotin bogs cell biology genes glycerol methanotrophs myo-inositol nitrogen-fixing bacteria nitrogenase nucleic acid hybridization nucleotide sequences peat peatlands ribosomal RNA Sphagnum sucrose |
Title | Isolation and characterization of nitrogen-fixing bacteria of the genus Azospirillum from the soil of a Sphagnum peat bog |
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